MICROASSAY METHODS FOR TAURINE AND CYSTEINE SULFINATE DECARBOXYLASE ACTIVITY
スポンサーリンク
概要
- 論文の詳細を見る
Microassay methods for taurine (2-aminoethanesulfonic acid) and cysteine sulfinate decarboxylase (CSD : EC 4.1.1.29) have been developed. Cation exchange resin (Dowex 50W × 8. 200-400 mesh) was used for the separation of taurine from other fluorescamine reactive substances. The “taurine fraction” in this column chromatographic procedure was collected and fluorescence development was carried out. The maximal sensitivity obtained for taurine was 25 pmoles. The specificity, reproducibility, sensitivity and recovery for taurine obtained by this method were satisfactory enough to be used for biological applications. The activity of CSD was determined by measuring the formation of <SUP>14</SUP>C0<SUB>2</SUB> from DL-[l-<SUP>14</SUP>C]-cysteine sulfinic acid using a specially designed micro-vessel. The activity in cerebral tissues containing 5 μg of protein was accurately detected by this microradiometrical method. Using above microassay methods for taurine and CSD activity, it was found that in the rat spinal cord, taurine is distributed rather evenly, whereas the distribution of CSD activities is uneven and the highest activity is localized at the dorsal part of the dorsal horn.
- 社団法人 日本薬理学会の論文
著者
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YONEDA YUKIO
Department of Pharmacology, Setsunan University
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Yoneda Yukio
Department Of Molecular Pharmacology Kanazawa University Faculty Of Pharmaceutical Sciences
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Kuriyama Kinya
Department Of Health Promoting Acupuncture And Moxibution Meiji University Of Oriental Medicine
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TAKASHIMA Sumie
Department of Pharmacology, Kyoto Prefectural University of Medicine
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HIRAI Kazuo
Department of Pharmacology, Kyoto Prefectural University of Medicine
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KURIHARA Etsuo
Department of Pharmacology, Kyoto Prefectural University of Medicine
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YUKAWA Yoshinao
Department of Pharmacology, Kyoto Prefectural University of Medicine
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TOKUNAGA Hisashi
Department of Pharmacology, Kyoto Prefectural University of Medicine
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YONEDA Yukio
Department of Pharmacology, Kyoto Prefectural University of Medicine
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