Release of Digestive Enzymes from Isolated Rat Pancreatic Acini Following Muscarinic Stimulation:A Comparative Study with Enzyme Release and Receptor Binding.
スポンサーリンク
概要
- 論文の詳細を見る
Effect of cholinergic stimulation on the release of digestive enzymes from isolated rat pancreatic acini prepared by collagenase digestion was investigated. The release of enzymes (amylase, chymotrypsinogen, lipase) increased linearly with the time of incubation in the absence of secretagogues. Carbachol, a muscarinic agonist, induced a remarkable increase in the release of these enzymes. This carbachol-stimulated amylase release showed a biphasic curve, and its maximal response was observed at 10<SUP>-5</SUP> M. The release patterns of chymotrypsinogen and lipase were similar to that of amylase. This carbachol-stimulated amylase release was inhibited by atropine in a dose-dependent manner, with an ED<SUB>50</SUB> value of 1.17 × 10<SUP>-8</SUP> M. Other cholinergic agonists such as methacholine also stimulated the release of these enzymes, and these increases in the release were inhibited by atropine. Scatchard analysis for [<SUP>3</SUP>H]quinuclidinyl benzilate ([<SUP>3</SUP>H]QNB) binding to isolated pancreatic acini revealed that the binding site of [<SUP>3</SUP>H]QNB was a single component with a K<SUB>d</SUB> value of 0.09 nM and a B<SUB>max</SUB> value of 89.3 fmol/mg protein, respectively. The effect of other cholinergic antagonists, pirenzepine and AF-DX 116 (11-[[2-[(diethylamino) methyl]1-piperidinyl]acetyl]-5, 11-dihydro-6H-pyrido[2, 3-b][1, 4]-benzodiazepine-6-one), on pancreatic acini was also investigated. Based on these results, it has been concluded that isolated rat pancreatic acini have muscarinic receptors and are useful for analyzing the mechanism of pancreatic enzyme secretion.
- 公益社団法人 日本薬理学会の論文
著者
-
KASHIMA Kei
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine
-
Kashima Kei
Third Department Of Internal Medicine And Kyoto Prefectural University Of Medicine
-
Kataoka Keisho
Third Department Of Internal Medicine Kyoto Prefectural University Of Medicine
-
Ohkuma Seitaro
Department Of Pharmacology Kawasaki Medical School
-
Kato Masato
Department Of Anesthesiology And Emergency Medicine Tohoku University Postgraduate Medical School
-
Kuriyama Kinya
Department Of Health Promoting Acupuncture And Moxibution Meiji University Of Oriental Medicine
-
Mukainaka Teruo
Laboratory of Electron Microscopy, Kyoto Prefectural University of Medicine
関連論文
- Low, but Physiological, Concentration of GLP-1 Stimulates Insulin Secretion Independent of the cAMP-Dependent Protein Kinase Pathway
- Double mutations of the N-ras gene in a patient with acute myelomonocytic leukemia
- Neurofibromatosis 1 gene (NF1) mutation is a rare genetic event in myelodysplastic syndrome regardless of the disease progression
- HEPATOBILIARY SARCOIDOSIS : TYPICAL APPEARANCE IN LAPAROSCOPY
- Clinicopathological Characteristics of Esophageal Squamous Papillomas in Japanese Patients : With Comparison of Findings from Western Countries
- ARGON PLASMA COAGULATION : IN VIVO TISSUE DAMAGE TO THE ESOPHAGUS AND STOMACH AND CLINICAL EFFICACY FOR EARLY ESOPHAGEAL AND GASTRIC CANCER
- Endoscopic resection for the diagnosis of visceral Kaposi's sarcoma
- Chronic diarrhea and protein-losing gastroenteropathy caused by Dientamoeba fragilis
- Increased QT Dispersion in Patients With Alcoholic Pancreatitis
- Molecular Cloning and Characterization of Chymopasin, a Novel Serine Protease from Rat Pancreas
- Ultrasonographic Splanchnic Arterial Flow Measurement in Severe Acute Pancreatitis
- Regression of atypical lymphoid hyperplasia after eradication of Helicobacter pylori
- Acupuncture Stimulates the Release of Serotonin, but Not Dopamine, in the Rat Nucleus Accumbens
- A case of gastic antral vascular ectasia - increase of neuroendocrine cells in the antrum
- Feasibility trial of adjuvant chemotherapy with paclitaxel and carboplatin after surgical resection in Japanese patients with non-small cell lung cancer : report of the Lung Oncology Group in Kyushu (LOGIK) protocol 0501
- Adenoendocrine cell carcinoma of the gallbladder clinically mimicking squamous cell carcinoma
- Ischemic hepatitis induced by mesenteric volvulus in a patient with chronic obstructive lung disease
- Expression of tumor-associated antigens in hepatocarcinogenesis : Immunocytochemical investigation of AFP and GGT
- Sepsis is Characterized by the Increases in Percentages of Circulating CD4^+CD25^+ Regulatory T Cells and Plasma Levels of Soluble CD25
- Biological Significance of AFP Expression in Liver Injury Induced by CCL_4
- Biological Significance of Gap Junctional Formation in Hepatoma Cells
- Activated hepatic stellate cells participate in liver fibrosis in a patient with transfusional iron overload
- Long-Term Organotypic Slice Culture of the Neonatal Mouse Liver
- Relation Between Morphologic Changes in the Main Pancreatic Duct and Exocrine Pancreatic Function after a Secretin Test
- Taurine attenuates the serum γ-GTP-decreasing effect of ursodeoxycholic acid in chronic hepatitis patients
- Ursodeoxycholic acid decreases the serum transaminase levels of chronic liver disease patients treated with glycyrrhizin
- Power spectral analysis of the electroencephalogram during induced total spinal block
- Celiac Plexus Block in Cancer Pain Management
- Comparison between in vivo and in vitro pharmacokinetics of succinylcholine in humans
- Neuromuscular Blocking Actions of the Aminoglycoside Antibiotics Sisomicin and Micronomicin in the Rabbit
- An Evaluation of Neuromuscular Reversal with Edrophonium in a Patient with Malathion Intoxication
- Factors influencing retention behavior of aluminum compounds on handsheets
- Hypervascular Liver Metastasis from Hypovascular Ductal Cell Carcinoma of the Pancreas
- Identification of apoptotic hepatocytes in situ in rat liver after lead nitrate administration
- The Expression of c-myc Messenger RNA during Rat Hepatocarcinogenesis
- Cell kinetic analysis of hyperplastic lesions appearing in early phase of hepatocarcinogenesis
- Irsogladine Maleate May Preserve Gastric Mucosal Hydrophobicity Against Ethanol in Phospholipids Independent Way in Rats
- Treatment With Bovine Gallstones Exacerbates Liver Damage, but Enhances Hepatoprotection by Bear Gall Powder in Carbon Tetrachloride-Intoxicated Rats
- Effectiveness of Bovine Gallstone (Goou) and Bear Gall Powder (Yutan) on Chronic Liver Diseases : A Preliminary Report
- Ischemic colitis during interferon-alpha treatment for chronic active hepatitis C
- How Do Acupuncture and Moxibustion Act? : Focusing on the Progress in Japanese Acupuncture Research
- Acute Liver Damage with Characteristic Apoptotic Hepatocytes by Ingestion of Aplysia kurodai, a Sea Hare
- Huge Mucinous Cystadenoma of the Pancreas Developing During Pregnancy : A Case Report
- First Phase of Glucose-Stimulated Insulin Secretion From MIN 6 Cells Does Not Always Require Extracellular Calcium Influx
- Intraoperative neurological monitoring in awake craniotomy
- THE β-ADRENERGIC RECEPTOR/ADENYLATE CYCLASE SYSTEM IN THE CARDIAC VENTRICLES OF A HYPERTROPHIC CARDIOMYOPATHY RAT MODEL
- Regeneration of Parietal Cells at the Healing Process of Acetic Acid-induced Gastric Ulcers in Rats : Evaluation of the Quality of Ulcer Healing by the Administration of Various Agents
- Early-stage blocking of Notch signaling inhibits the depletion of goblet cells in dextran sodium sulfate-induced colitis in mice
- The effect of steroid pulse therapy on the development of acute pancreatitis induced by closed duodenal loop in rats
- Pathophysiologic Studies of Experimental Chronic Pancreatitis in Rats Induced by Injection of Zein-Oleic Acid-Linoleic Acid Solution into the Pancreatic Duct
- Diagnosis of Chronic Pancreatitis Using Noninvasive Tests of Exocrine Pancreatic Function-Comparison to Duodenal Intubation Tests
- Extrahepatic biliary cystadenocarcinoma arising from the left hepatic duct
- Stimulated Kupffer cells attract cytotoxic T lymphocytes (CTL) and increase the adhesion of CTL to sinusoidal endothelial cells
- Regional Differences of L-type High Voltage-Gated Calcium Channel Subunit Expression in the Mouse Brain After Chronic Morphine Treatment
- Increase in Expression of α1 and α2/δ1 Subunits of L-Type High Voltage-Gated Calcium Channels After Sustained Ethanol Exposure in Cerebral Cortical Neurons
- Effects of etodolac, a selective cyclooxygenase-2 inhibitor, on the expression of E-cadherin-catenin complexes in gastrointestinal cell lines
- Unusual Multiple Gastric Carcinoids with Hypergastrinemia : Report of a Case
- ROLES OF BIOSYNTHESIZED AND TRANSPORTED TAURINE AND GLYCINE IN THE RORMATION OF CONJUGATED BILE ACIDS IN HEPATOCYTES
- Anesthetics, immune cells, and immune responses
- Functional Proteins Involved in Regulation of Intracellular Ca^ for Drug Development : Chronic Nicotine Treatment Upregulates L-Type High Voltage-Gated Calcium Channels
- MICROASSAY METHODS FOR TAURINE AND CYSTEINE SULFINATE DECARBOXYLASE ACTIVITY
- Role of Nitric Oxide in Central Synaptic Transmission: Effects on Neurotransmitter Release
- Functional Proteins Involved in Regulation of Intracellular Ca2+ for Drug Development: Chronic Nicotine Treatment Upregulates L-Type High Voltage-Gated Calcium Channels
- PURIFICATION OF γ-AMINOBUTYRIC ACID (GABA)/BENZODIAZEPINE RECEPTORS USING BENZODIAZEPINE-AFFINITY COLUMN CHROMATOGRAPHY : BIOCHEMICAL AND PHARMACOLOGICAL PROPERTIES OF PURIFIED RECEPTORS
- Expression of Serotonin Transporter in Mice With Ethanol Physical Dependency
- Expression of Serotonin Transporter in Mice With Ethanol Physical Dependency
- Stress Induced Alterations in Striatal GABAA Receptor Complex
- Pseudo-Meigs' Syndrome Associated with Breast Cancer Metastasis to Both Ovaries : Report of a Case
- STUDIES ON β-PYRIDYLCARBINOL AS A PRECURSOR OF NICOTINAMIDE ADENINE DINUCLEOTIDE
- A SIMPLE AND RAPID MICROASSAY METHOD FOR ADENOSINE USING COMBINED EFFECTS OF ISOTOPE DILUTION AND ENZYMATIC CATALYSIS
- Inhibition of human plasma cholinesterase and erythrocyte acetylcholinesterase by nondepolarizing neuromuscular blocking agents
- EFFECT OF ETHANOL ADMINISTRATION ON GUANYLATE CYCLASE ACTIVITIES IN LIVER AND BRAIN
- ALTERATION OF DOPAMINE AND TRH IN RAT BRAIN DURING HYPOTHERMIA
- PRETREATMENT WITH TAURINE FACILITATES HEPATIC LIPID PEROXIDE FORMATION ASSOCIATED WITH CARBON TETRACHLORIDE INTOXICATION
- ROLE OF CALCIUM IN HEPATIC LIPID PEROXIDE FORMATION ASSOCIATED WITH CARBON TETRACHLORIDE-INTOXICATION
- ALTERATION OF HEPATIC LIPOGENESIS AND LIPOLYSIS IN PERIPHERAL ADIPOSE TISSUES FOLLOWING ACUTE AND CHRONIG ALCOHOL ADMINISTRATIONS
- CHARACTERISTICS OF TAURINE TRANSPORT IN FRESHLY ISOLATED RAT HEPATOCYTES
- EFFECT OF PCB (POLYCHLOROBIPHENYLS) ON L-ASCORBIC ACID, PYRIDOXAL PHOSPHATE AND RIBOFLAVIN CONTENTS IN VARIOUS ORGANS AND ON HEPATIC METABOLISM OF L-ASCORBIC ACID IN THE RAT
- Oxidative stress is closely associated with tumor angiogenesis of hepatocellular carcinoma
- Metastatic Breast Carcinoma Simulating Linitis Plastica of the Colon : Report of a Case
- THERAPEUTIC EFFECT OF TAURINE ADMINISTRATION ON CARBON TETRACHLORIDE-INDUCED HEPATIC INJURY
- AN IMPROVED GAS-LIQUID CHROMATOGRAPHIC METHOD USING SILICON AN-600 COLUMN FOR SEPARATION OF BILE ACIDS: ITS APPLICATION ON ANALYSIS OF BILE ACIDS IN RAT BILE
- EFFECT OF ORGANOPHOSPHORUS COMPOUNDS ON ACETYLCHOLINE SYNTHESIS IN BRAIN
- EFFECT OF TAURINE ON ALTERATION IN ADRENAL FUNCTIONS INDUCED BY STRESS
- Nuclear size measurement is a simple method for the assessment of hepatocellular aging in non-alcoholic fatty liver disease : Comparison with telomere-specific quantitative FISH and p21 immunohistochemistry
- QT intervals and QT dispersion in patients with subarachnoid hemorrhage
- Dopamine D_1 Receptors Participate in Cocaine-Induced Place Preference via Regulation of Ryanodine Receptor Expression
- Increase in Expression of α1 and α2/δ1 Subunits of L-type High Voltage-Gated Calcium Channels After Sustained Ethanol Exposure in Cerebral Cortical Neurons
- HEPATIC LIPOGENESIS AND MOBILIZATION OF PERIPHERAL FATS IN THE FORMATION OF ALCOHOLIC FATTY LIVER
- Effect of Aripiprazole on Anxiety Associated With Ethanol Physical Dependence and on Ethanol-Induced Place Preference
- Acamprosate Suppresses Ethanol-Induced Place Preference in Mice With Ethanol Physical Dependence
- Prognosis in Adult Patients with Idiopathic Pulmonary Hemosiderosis
- Insulin facilitates formation of taurine-conjugated bile acids - Analysis using primary cultured hepatocytes.
- Syntheses of new optically active organosilanes and their applications for the optical-purity-determining agents.
- Release of Digestive Enzymes from Isolated Rat Pancreatic Acini Following Muscarinic Stimulation:A Comparative Study with Enzyme Release and Receptor Binding.
- Regulatory Mechanisms and Pathophysiological Significance of IP3 Receptors and Ryanodine Receptors in Drug Dependence
- Roles of endogenous and exogenous taurine and glycine in the formation of conjugated bile acids: Analyses using freshly isolated and primary cultured rat hepatocytes.
- Pharmacological characteristics of choline transport system in mouse cerebral cortical neurons in primary culture.
- Muscimol Prevents Neuronal Injury Induced by NMDA.
- Inhibition of Gastric Glucosamine Synthetase Activity by Oxygen Radicals: A Possible Cause of Decreased Mucosal Protective Capacity.