A NEW MICROASSAY METHOD FOR L-GLUTAMIC ACID DECARBOXYLASE (GAD) ACTIVITY
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概要
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A simple and sensitive microradiometrical assay method for L-glutamic acid decarboxylase (GAD: EC 4.1.1.15) has been designed. Cerebral tissue was frozen and sectioned at 300 μ thickness, after which the slice was further dissected into rectangular blocks (750 750 μ). The GAD activity was assayed microradio-metrically. The incubation vessel held a volume of approximately 500 μl and the incubation mixture was 90 μl. The <SUP>14</SUP>CO<SUB>2</SUB> evolved from L-[l-<SUP>14</SUP>C]-glutamic acid was absorbed with a filter paper immersed in 100 μl of hyamine base. GAD activity in neuronal tissues containing less than 5-10 μg of protein was easily and accurately detected by this method. The assay was found to be linear at 5-500 μg of protein concentration in various CNS preparations. Utilizing this microradiometric assay method, it was found that in the rat hypothalamus, the distribution pattern of GAD activity was essentially the same as that of GABA.
- 社団法人 日本薬理学会の論文
著者
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Kuriyama Kinya
Department Of Health Promoting Acupuncture And Moxibution Meiji University Of Oriental Medicine
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Kimura Hiroshi
Department Of Anatomy Shiga University Of Medical Science
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Kimura Hiroshi
Department Of Anatomy Ii Shiga University Of Medical Science
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KIMURA Hiroshi
Department of Pharmacology, Kyoto Prefectural University of Medicine
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