PURIFICATION OF HISTAMINE RECEPTOR : (II) SUBCELLULAR DISTRIBUTION OF SPECIFICALLY BOUND DIBENAMINE

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By means of differential centrifugation and density gradient separation, fractionation of histamine receptors of cat small intestinal smooth muscle was undertaken. The radioactivity differences were investigated between the muscle labeled with radioactive dibenamine under drug protection and the muscle without the protective drug. The fraction was obtained in which specifically labeled histamine receptors were concentrated. The specificity of the fraction was proved by examining the pattern of distribution of radioactivity differences in subcellular fractions of the muscle protected with specific drugs (diphenhydramine, chlorpheniramine and histamine) or with non-specific drugs (aniline, phenethylamine and benzylamine). The labeled components were of high molecular weight and degradated to smaller molecules by solubilization of the fraction with Triton X-100. A good correlation was found between radioactivity differences and pharmacological observations.
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