PURIFICATION OF HISTAMINE RECEPTOR (VI) AN IMPROVED DOUBLE LABELING METHOD WITH “DOUBLE PROTECTION”
スポンサーリンク
概要
- 論文の詳細を見る
Studies were done on the specific labeling of the histaminergic H<SUB>1</SUB>-receptor of the longitudinal smooth muscle of cat small intestine. A procedure involving ‘double protection’ combined with the double labeling technique was developed. The first protection was the usual with a protective antihistamine, promethazine, and the second was cross protection of non-specific sites with non-hitaminergic drugs, thioriazine and atropine. Muscle tissue protected with promethazine against non-radioactive dibenamine was treated with <SUP>3</SUP>H-dibenamine in the presence of these second protectors. The second protectors covered non-receptor sites which had been protected from non-radioactive dibenamine with promethazine. The dose-response curves were carefully checked in each experiment to confirm that the second protectors did not interfere with the specific coverage provided by the first protector. Finally <SUP>14</SUP>C-dibenamine was applied to measure non-specific binding after which the labeled muscles were fractionated and the radioactivity was counted. The specificity of labeling achieved in the receptor-rich fraction by this method is discussed.
- 社団法人 日本薬理学会の論文
著者
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UCHIDA Masaatsu
Department of Pharmacology, Meiji Pharmaceutical University
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Uchida Masaatsu
Department Of Molecular Pharmacology Meiji College Of Pharmacy
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UCHIDA Masaatsu
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo
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