Datura stramonium Agglutinin Released Histamine from Rat Peritoneal Mast Cells That Was Inhibited by Pertussis Toxin, Haptenic Sugar and N-Acetylglucosamine-Specific Lectins: Involvement of Glycoproteins with N-Acetylglucosamine Residues.
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概要
- 論文の詳細を見る
The <I>N</I>-acetyl glucosamine (GlcNAc)-specific lectin <I>Datura stramonium</I> agglutinin (DSA) rapidly and sugar-specifically released histamine from rat peritoneal mast cells, and pertussis toxin (IAP) inhibited it, suggesting that DSA activated mast cells via an IAP-sensitive G protein pathway. The additive effects of DSA and basic secretagogues such as compound 48/80 that activate IAP-sensitive G protein directly suggest that they shared the same mechanism of action including involvement of the IAP-sensitive G protein. Using lectin-blotting, blots of the corresponding glycoproteins detected by DSA diminished by haptenic sugar or pretreatment of the cells with <I>N</I>-glycosidase F, suggesting that the binding of DSA was responsible for the mast cell activation. The other GlcNAc-specific lectins such as <I>Phytolacca americana</I> mitogen, <I>Solanum tuberosum</I> agglutinin and wheat germ agglutinin (WGA) inhibited the histamine release induced by DSA, suggesting that these lectins were antagonists, but DSA was an agonist. Sialic acid-specific <I>Macckia</I> <I>amurensis</I> mitogen (MAM) inhibited the histamine release, and neuraminidase-treatment decreased mast cell activation induced by DSA. At least four mast cell glycoproteins that have affinity to DSA, WGA and MAM and are sensitive to neuraminidase-treatment were detected by lectin-blotting. Some of them may be binding sites coupled to histamine release including the IAP-sensitive G protein pathway. DSA is a useful tool for studying signal transduction of mast cells including the involvement of the IAP-sensitive G protein.
- 公益社団法人 日本薬理学会の論文
著者
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Matsuda Koji
Department Of Endoscopy Jikei University
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UCHIDA Masaatsu
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo
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SUZUKI-NISHIMURA Tamiko
Department of Molecular Pharmacology, Meiji College of Pharmacy
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Matsuda Koji
Department of Molecular Pharmacology, Meiji College of Pharmacy
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Aoki Junken
Tokyo Metropolitan Institute of Medical Sciences
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