Inhibition of Soluble-Aβ1-42 Oligomer Formation by Novel Peptides

概要

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There have been many reports suggesting that soluble oligomers of amyloid β (Aβ) are neurotoxins causing Alzheimers disease (AD). Although inhibition of the soluble oligomerization of Aβ is considered to be effective in the treatment of AD, almost all peptide inhibitors have been designed from the β-sheet structure (H14-D23) of Aβ1-42. To obtain more potent peptides than the known inhibitors of the soluble-oligomer formation of Aβ1-42, we performed random screening by phage display. After fifth-round panning of a hepta-peptide library against soluble Aβ1-42, novel peptides containing arginine residues were enriched. These peptides were found to suppress specifically 37/48 kDa oligomer formation and to keep the monomeric form of Aβ1-42 even after 24 h of incubation, as disclosed by SDS-PAGE and size-exclusion chromatography. Thus we succeeded in acquiring novel efficient peptides for inhibition of soluble 37/48 kDa oligomer formation of Aβ1-42.