高リン食摂取ラットの腎におけるシトクロームp450, HSP-70, HO-1, iNOSの発現

概要

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Five-week-old rats were fed on a diet containing phosphorus either at 0.3% (control diet) or at 1.5% (high P diet) for 21 days. Mitochondrial, microsomal, cytosol fractions were obtained from the kidney by the usual method. Kidney of rat fed on high P diet contained high concentration of calcium, and its function was impaired because of excessive calcium. In this case, many stress defensive proteins like cytochrome p-450, HSP-70, HO-1, iNOS in kidney seem to appear. Then those proteins in those fractions were detected by the western blotting method. Contents of cytochrome p-450, HSP-70 and iNOS in three fractions were almost the same between in control diet rats and high P diet rats. On the other hand, HO-1 was not detected in three fractions of control rats, but it was detected in high P rats. HO-1 may play some defensive roles in environmental condition, that is, the cellular high calcium concentration.
武庫川女子大学の論文
2001-03-31