Formation of Deoxyshikonin in Callus Cultures of Lithospermum erythrorhizon

概要

論文の詳細を見る
Callus tissues of Lithospermum erythrorhizon Sieb. et Zucc. are capable of synthesizing shikonin derivatives including acetyl-, β-hydroxyisovaleryl-, and isovaleryl-shikonin when cultured in the dark. The present paper describes that callus tissues grown in the dark also form deoxyshikonin, which is a shikonin derivative isolated recently from the roots of L. erythrorhizon and Macrotomia euchroma by Kyogoku et al. A red pigment, which corresponded to deoxyshikonin in its chromatographic behavior on TLC, was isolated from the CHCl_3 extract of callus tissues. This compound was identified as deoxyshikonin by direct comparison with authentic sample by mixed mp, TLC, UV and IR spectra. The content of deoxyshikonin in callus tissues was approximately 0.02 % per dry weight of tissue, which is comparable to 0.016 % in the intact roots. By contrast, no deoxyshikonin was detectable in callus tissues irradiated with white or blue light during the culture period.
日本生薬学会の論文
1974-06-20