A Simple and Rapid Protocol for Preparation of Crude Drug DNA Suitable for PCR
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概要
- 論文の詳細を見る
A protocol for rapid purification of DNA from a variety of herbal crude drugs, starting from samples as small as 1 mg, has been developed. Neither phenol extraction nor alcohol precipitation is involved in the procedure. Total nucleic acids are first extracted with the lysis buffer containing SDS. After CHCl_3 extraction, DNA is bound to glass fiber fleece in the presence of guanidine thiocyanate, while unbound impurities are washed away. The entire procedure can be carried out in 1.5-ml microtubes. The purified DNA obtained serves as a template for PCR.
- 公益社団法人日本薬学会の論文
- 1999-07-15
著者
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Mizukami Hajime
Faculty Of Pharmaceutical Science Nagoya City University
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Okabe Y
Nagoya City Univ. Nagoya Jpn
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Okabe Yuka
Faculty Of Pharmaceutical Sciences Nagoya City University
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