85. 12位の水酸化から始まるジャスモン酸イソロイシンンの代謝に関する研究(口頭発表,植物化学調節学会第46回大会)

概要

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Jasmonate is plant hormone that plays crucial roles in development and defense response. It has been shown that 7-iso-jasmonyl-L-isoleucine (JA-Ile) is the bioactive form involved in the jasmonate-mediated signaling pathway. The hormonal action of jasmonate in plants is controlled by the precise balance between its biosynthesis and catabolism. Recently, CYP94B3, JA-Ile 12-hydroxylase was considered one of important enzymes that played fine tuning system of active form. Here, we studied the metabolic pathway of JA-Ile started with 12-hydroxylation. Firstly, we synthesized 12-carboxy1JA-Ile and 12-O-D-β-glucopyranosy1JA-Ile, which were expected metabolites of 12-hydroxyJA-Ile. Secondly, the endogenous levels of the compounds in wounded leaves of wild type or cyp94b3 mutant were analyzed by UPLC-MS/MS. Both compounds accumulated and the amount of 12-O-D-β-glucopyranosy1JA-Ile in cyp94b3 mutant was lower than that in wild type. In other hands, the 12-carboxy1JA-Ile level was same in wildtype and cyp94b3 mutant. This result suggests 12-carboxy1JA-Ile is synthesized by enzyme different from CYP94B3. Finally, we investigated 12-hydroxyJA-Ile glucosyltransferase. The enzyme which converted 12-hydroxyJA to 12-O-D-P-glucopyranosy1JA also had activity when 12-hydroxyJA-Ile was used as substrate. Currently, we survey the biological activity of 12-carboxy1JA-Ile and 12-O-D-13-glucopyranosy1JA-Ile.
2011-10-03