84. UDP-glucose非依存性イネ由来糖転移酵素に関わる糖供与体の探索(口頭発表,植物化学調節学会第46回大会)

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Plant hormone, jasmonic acid (JA), has been recognized as a signal molecule involved in the wounding response. But two metabolites of JA, tuberonic acid (TA; 12-hydroxyjasmonic acid) and its β-glucoside (TAG) have not been reported to show any clear physiological function on the wounding response. In order to establish functions of these compounds, we previously purified and characterized a glucosyltransferase in rice, that catalyzes the glucosylation from TA to TAG using UDPG as a glucose donor [1]. Moreover, we found out the existence of UDPG-independent one in the crude enzyme, that is more specific to TA than that one. In order to understand this glucosylation, we investigated a glucose donor except UDPG in this study. To test an activity of glucosyltranslation, we carried out TA glucosyltransferase assay using a crude enzyme in the rice cell culture. We used TA as a substrate and analyzed resulting TAG by UPLC-MS/MS. Using this assay system, an 80% ethanol extract derived from rice cell cultures (376.3 g) was purified by several series of column chromatography, and finally, compound 1 (1.5 mg) was obtained. The chemical structure of compound 1 is determined by NMR analysis that is to be 3-(4-hydroxyphenyl)prop-2-enyl β-D-glucopyranoside. This was synthesized from p-coumaric acid as starting compound, and the synthesized compound also transferred glucose to TA. Therefore, we concluded that compound 1 is a natural glucose donor in the rice cell culture.
2011-10-03

84. UDP-glucose非依存性イネ由来糖転移酵素に関わる糖供与体の探索(口頭発表,植物化学調節学会第46回大会)

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