62.ツベロン酸グルコシルトランスフェラーゼの精製とその生理的作用に関する研究(口頭発表)

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Tuberonic acid (12-hydroxy epi-jasmonic acid, TA) and its glucoside (TAG) were isolated from potato leaflets (Solanum tuberosum L.) and shown to have tuber-inducing properties. These compounds are known to be biosynthesized by an octadecanoid pathway. The metabolism of jasmonic acid (JA) to TAG in plant leaflets and translocation of the resulting TAG to the distal parts was demonstrated. It is thought that TAG generated from JA transmits a wounded signal from damaged parts to undamaged parts by this mechanism. The metabolism of TA in higher plants was demonstrated using [12-^3H] TA, and TA glucosyltransferase was purified from rice cell culture. The purified protein was shown to be a possible salicylic acid (SA) glucosyltransferase (OsSGT) by MALDI-TOF-MS analysis. Recombinant OsSGT obtained by overexpression in Escherichia coli was active toward TA but also toward SA. The OsSGT characterized in this research was not specific, but this is the first report of TA glucosyltransferase. mRNA expressional analysis of OsSGT purified in this study and quantification of TA, TAG, SA and SAG after mechanical wounding indicated that OsSGT is involved in the wounding response. Tobacco SA glucosyltransferase was also active toward TA, and the mRNA expression was also induced by the wounding stress. These results demonstrated the crucial role of TAG not only in potato tuber formation, but also in the stress response in plants.
植物化学調節学会の論文
2008-10-06

62.ツベロン酸グルコシルトランスフェラーゼの精製とその生理的作用に関する研究(口頭発表)

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