41. シロイヌナズナ・アクセッションにおける種子休眠性と植物ホルモン内生量の網羅的解析(口頭発表,植物化学調節学会第46回大会)

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Seed dormancy, defined as the failure of viable seed to germinate under favorable conditions, is thought to be an important factor that determines geographical distribution of plants. Increasing lines of evidences have demonstrated that phytohormones, such as abscisic acid (ABA) and gibberellins (GA), play an essential role in seed dormancy and germination. In Arabidopsis, it is widely known that wild-type accessions, which originate from various regions of the world, significantly differ in the degree of seed dormancy. Cvi-0, one of dormant accession, has been reported to show altered endogenous ABA/GA balance in agreement with the reduced germination frequencies. In this study, we analyzed seed germination and phytohormone levels in the 2-months after-ripened seeds of over 100 accessions. Analysis of 6 phytohormones including ABA and GA first revealed that there are significant variations in all horomone levels among these accessions. Interestingly, endogenous levels of ABA, GA and isopentenyladenine (iP) levels after seed imbibition showed negative (ABA) and positive (GA, iP) correlation with germination frequencies among accessions, suggesting the universal role of phytohormone balance in seed dormancy and germination. To identify genetic factors that are associated with these phenotypic variations, we next performed genome-wide association mapping analysis. Several candidate genes that would potentially explain the phenotypic variations have been identified so far.
2011-10-03

41. シロイヌナズナ・アクセッションにおける種子休眠性と植物ホルモン内生量の網羅的解析(口頭発表,植物化学調節学会第46回大会)

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