83 シロイヌナズナ種子発芽過程における内生ジベレリンとアブシジン酸

概要

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We have previously identified GA-responsive genes using oligonucleotide-based DNA microarray in GA-treated and non-treated gal-3 mutant seeds. In this study, we examined whether expression of these GA-responsive genes (identified in the gal-3 mutant) correlates with changes in the levels of endogenous GAs and/or abscisic acid (ABA) during wild-type seed germination. The levels of bioactive GAs, GA_1 and GA_4,gradually increased when wild-type seeds were incubated at 22℃ in the light. ABA levels were drastically decreased immediately after the start of imbibition. Gene expression profiles in germinating wild-type seeds indicated that most of the GA-inducible genes identified in the gal-3 mutant were upregulated upon imbibition of wild-type seeds, which may correlate with an increase in the amount of endogenous GAs. We also analyzed the correlation between mRNA levels of GA biosynthesis genes and levels of endogenous GAs. This comparison allows us predict key GA biosynthesis genes by determination of GA content in germinating Arabidopsis seeds.
植物化学調節学会の論文
2002-10-31