2A2-N05 微生物のトラッキングと蛍光観察を組合せた顕微鏡システムの開発(ナノ・マイクロバイオシステム)

概要

論文の詳細を見る
Molecular biologists use a fluorescence microscope to observe intracellular ions (molecules) and study intracellular mechanisms realizing cellular functions. However, they often miss a freely moving cell from a scope of the fluorescence microscope and then discontinue observation. To overcome this problem, we developed a new microscope system, which enables us to observe intracellular ions and molecules with a fluorescent light and track the cell with a transmitted light at the same time. High-speed visual feedback control was an efficient technique for cell tracking. We demonstrated that it is possible to continuously observe intracellular Ca2+ ions of the freely moving paramecium, which is one of the fastest cells. Our microscope system should become an irreplaceable tool to investigate a frontier in molecular biology.
一般社団法人日本機械学会の論文
2007-05-11