急性の実験的精巣捻転ラットにおけるAkt/Protein Kinase BとExtracellular Signal-regulated Kinaseの活性化(解剖学)
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概要
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Akt/Protein Kinase B(PKB)とExtracellular Signal-Regulated Kinase(ERK)経路は細胞の生存に関与する.本研究では,血管結紮/再灌流(I/R)後のラット精巣におけるAkt/PKBの一過性局在とERK1/2の活性化について報告する.乏血障害後6, 24, 48時間再灌流した精巣組織ならびに正常の精巣組織を採取し,ウエスタンブロットならびに免疫組織化学的解析を行った.再灌流6-24時間で,リン酸化Akt/PKB(pAkt/PKB)ならびにERK1/2(pERK1/2)レベルが顕著に増加した.しかし,再灌流48時間で,これらの活性化蛋白は徐々に減少した.免疫組織化学的に,正常精巣のセルトリ細胞でpAkt/PKBの弱い発現が見られた. I/R後, pAkt/PKB発現はセルトリ細胞ならびに精子形成細胞の管腔反対側で主に増加した.加えて,pERK1/2発現は正常精巣ではセルトリ細胞ならびにライディッヒ細胞に観察され, I/R後のpERK1/2発現は生存中の精子形成細胞(主に精母細胞)とセルトリ細胞管腔反対側で増加した.これらの結果は, Akt/PKBとERKが精巣I/R術初期において精巣細胞の生存に関与することを示す.これらの経路は,精巣捻転を含む精巣障害において多くの細胞を生存させるために重要な指標となるだろう.
- 2008-04-25
著者
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Kim Jong-choon
Coll. Of Veterinary Medicine Chonnam National Univ.
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Kim Sung
韓国
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SHIN Taekyun
Department of Veterinary Medicine, Graduate School, Cheju National University
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KIM Seungjoon
Department of Veterinary Medicine, Graduate School, Cheju National University
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MOON Changjong
Department of Veterinary Medicine, College of Applied Life Science and Applied Radiological Science
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Lee Hae-june
Department Of Veterinary Anatomy College Of Veterinary Medicine Chonnam National University
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Lee Y
Chungnam National Univ. Kor
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LEE Yongduk
Department of Veterinary Anatomy, College of Veterinary Medicine and Research Institute for Subtropi
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KIM Joong-Sun
Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Cen
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KANG Seong
Department of Veterinary Surgery, College of Veterinary Medicine and Veterinary Medical Research Cen
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BAE Chun-Sik
Department of Veterinary Surgery, College of Veterinary Medicine and Veterinary Medical Research Cen
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JANG Hyosun
Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University
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KIM Sung-ho
Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University
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KIM Jong-Choon
Department of Veterinary Toxicology, College of Veterinary Medicine, Chonnam National University
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Kim Seungjoon
Department Of Veterinary Anatomy College Of Veterinary Medicine Chonnam National University
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Jang Hyosun
Department Of Veterinary Anatomy College Of Veterinary Medicine Chonnam National University
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Bae Chun-sik
Department Of Veterinary Surgery College Of Veterinary Medicine Chonnam National University
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Sim Ki
Department Of Neurosurgery College Of Medicine Cheju National University
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Kim Jong-choon
Department Of Pharmacology And Toxicology College Of Veterinary Medicine Chonnam National University
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Kang Seong
Department Of Veterinary Surgery College Of Veterinary Medicine Chonnam National University
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Kang Seong
Department Of Chemistry And Research Institute Of Physics And Chemistry (rinpac) Chonbuk National Un
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Lee Youngho
Department Of Physiology College Of Medicine Yonsei University
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Lee Yongduk
Department Of Veterinary Medicine Cheju National University
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Shin Taekyun
Jeju National Univ. Jeju
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Shin Taekyun
Department Of Veterinary Medicine Cheju National University
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Moon C
Department Of Veterinary Anatomy College Of Veterinary Medicine Chonnam National University
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Moon Changjong
Department Of Physiology University Of The Ryukyus School Of Medicine:department Of Veterinary Medic
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Shin T
Jeju National Univ. Jeju
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Kim Sung-ho
Department Of Biology Kongju National University
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Shin Taekyun
Department of Veterinary Anatomy, College of Veterinary Medicine and Research Institute for Subtropical Agriculture and Biotechnology, Cheju National University
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Kang Seong
Department of Advanced Materials Engineering for Information and Electronics, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea
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SHIN Taekyun
Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University
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