Gamma-Ray Susceptibility of Immature and Mature Hippocampal Cultured Cells
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概要
- 論文の詳細を見る
Ionizing radiation suppresses neurogenesis in the mammalian brain. This in vitro study compared the detrimental effect of acute gamma-irradiation on immature hippocampal cells with mature cells. Both rat immature [0.5 day in vitro (DIV)] and mature hippocampal cells (14 DIV) were irradiated with 0–4 Gy gamma-rays. Cell viability was analyzed by using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. DNA fragmentation study was performed by extracting intracellular DNA. Morphological features of apoptosis were characterized by 4,6-diamidine-2-phenylindole, dihydrochloride (DAPI) staining. MTT assay revealed that the survival rate of immature hippocampal cells declined in a dose-dependent manner within the range of irradiation applied, but was not changed in mature cells. Intranucleosomal DNA fragmentation in a ladder like pattern was dose-dependently increased in immature cells, but not in mature cells. The number of apoptotic nuclei in immature cells increased significantly in a dose-dependent manner within the range of irradiation applied. Active caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP) expressions in immature hippocampal cells at 6 hr after 2 Gy exposure were markedly higher than control levels. The significantly greater radiosensitivity of immature hippocampal cells than that of the mature cells, indicates that the susceptibility of such hippocampal cells depends on their maturation. In addition, gamma-irradiation may induce caspase-dependent apoptosis in immature hippocampal cells.
- 社団法人 日本獣医学会の論文
著者
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Kim Jong-choon
Coll. Of Veterinary Medicine Chonnam National Univ.
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Kim Jong-Choon
College of Veterinary Medicine, Chonnam National University
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Kim Sung-ho
College Of Veterinary Medicine Chonnam National University
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Kim Jong-choon
College Of Veterinary Medicine Chonnam National University
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SONG Myoung-Sub
College of Veterinary Medicine and Animal Medical Institute, Chonnam National University
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KIM Joong-Sun
College of Veterinary Medicine and Animal Medical Institute, Chonnam National University
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YANG Miyoung
College of Veterinary Medicine and Animal Medical Institute, Chonnam National University
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PARK Soo
College of Veterinary Medicine and Animal Medical Institute, Chonnam National University
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SHIN Taekyun
College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National Un
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MOON Changjong
College of Veterinary Medicine and Animal Medical Institute, Chonnam National University
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