Cloning and Nucleotide Sequence of Thermostable Lipase Gene from Pseudomonas fluorescens SIK W1(Biological Chemistry)
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概要
- 論文の詳細を見る
A gene coding for a thermostable lipase of Pseudomonas fluorescens SIK W1 was cloned into Escherichia coli JM83 by inserting Sau3AI-generated DNA fragments into the BamHI site of pUC19. Twenty colonies with esterase activity on teh tributyrin agar plate were isolated by screening the constructed pseudomonas fluorescens genomic library. Onlt one out of the esterase positive 20 colonies had lipase activity on the agar plate containing olive oil and Rhodamine-B. The complete nucleotide sequence of the lipase gene was identified. The lipase gene consists of an open reading frame, 1347bp long, commencing with an ATG start codon encoding a polypeptide of 449 amino acid residues and a TGA stop codon. Comparison of this lipase amino acid sequence with those from another organisms sequenced to data showed the presence of the short homologous region Gly-X-Ser-X-Gly.
- 社団法人日本農芸化学会の論文
- 1991-09-23
著者
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Rhee J
Korea Advanced Inst. Sci. And Technol. Taejon Kor
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Jeohn Gwang
Department Of Biological Scienece And Engineering Korea Advanced Institute Of Science And Technology
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Lee Young
Department Of Aerospace Information Engineering The Konkuk University
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Yoo O
Department Of Life Science Korea Advanced Institute Of Science And Technology
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Yoo Ook
Department Of Biological Science
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Lee Young
Department Of Biological Scienece And Engineering Korea Advanced Institute Of Science And Technology
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Rhee Joon
Department Of Biological Science And Engineering Korea Advanced Institute Of Science Of Technology
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CHUNG Guk
Department of Biological Scienece and Engineering Korea Advanced Institute of Science and Technology
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RHEE Joon
Department of Biological Science & Engineering
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