Cloning and Nucleotide Sequence of an Esterase Gene from Pseudomonas fluorescens and Expression of the Gene in Escherichia coli(Biological Chemistry)
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概要
- 論文の詳細を見る
A gene coding for a novel esterase of Pseudomonas fluorescens was cloned in this study. DNA sequencing showed that the open reading frame is comprised of 708 nucleotides. The coding sequence of the gene is preceded by a potential Shine-Dalgarno sequence and by a promoter-like structure. Following the stop codon a structure reminiscent of the E. coli rho-independent terminator is present. The enzyme expressed in an E. coli clone was mostly in the periplasmic space, released to the outside of the cell by osmotic shock and purified to homogeneity by QAE-Sephadex A-50 and DEAE-Sepharose columns. The native form of the enzyme consisted of two identical subunits, each with a molecular weight of 27,000. By studying the properties and substrate specificity, the enzyme was classified as an arylesterase (EC 3.1.1.2).
- 社団法人日本農芸化学会の論文
- 1990-08-23
著者
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CHOI Kang
Department of Biotechnology, Yonsei University
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Choi K
Korea Advanced Inst. Science And Technology Seoul Kor
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Rhee J
Korea Advanced Inst. Sci. And Technol. Taejon Kor
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Jeohn Gwang
Department Of Biological Scienece And Engineering Korea Advanced Institute Of Science And Technology
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RHEE Joon
Department of Biological Science and Engineering, The Korea Advanced Institute of Science
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YOO Ook
Department of Biological Science and Engineering, The Korea Advanced Institute of Science
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Choi Kang
Department Of Biological Science And Engineering Korea Advanced Institute Of Science And Technology
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Yoo O
Department Of Life Science Korea Advanced Institute Of Science And Technology
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Yoo Ook
Department Of Biological Science
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Rhee Joon
Department Of Biological Science And Engineering Korea Advanced Institute Of Science Of Technology
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RHEE Joon
Department of Biological Science & Engineering
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