Measurement of Superoxide Dismutase-like Activity of Natural Antioxidants

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概要

• 論文の詳細を見る

• The superoxide dismutase (SOD)-like activity of natural antioxidants was evaluated by measuring the inhibition of pyrogallol autoxidation that is catalyzed by the superoxide radical. Among 22 water-soluble antioxidants tested, L-ascrobic acid, L-ascorbic acid 6-palmitate, glutathione (reduced form), (+)-catechin, and (-)-epicatechin showed effective SOD-like activity. To analyze lipophilic antioxidants, an optically clear organic system composed of diethyl ether, surfactant (dioctyl sulfosuccinate, AOT) and water, called reverse micelles, was developed. The optimum concentrations of AOT, water and pyrogallol for determining SOD-like activity were found to be 50mM, 1.3M, and 40mM, respectively. After proving that pyrogallol autoxidation was mediated by the superoxide anion in that system, the SOD-like activity of 24 lipophilic antioxidants was measured. Cinnamon oil, γ-oryzanol, extract of rosemary leaf, L-α-lecithin, and L-α-cephalin exhibited activity, although the activity of some antioxidants could not be measured because of the intense color or low solubility.

• 社団法人日本農芸化学会の論文
• 1995-05-23

著者

• Kim Seok

  Department of Obstetrics and Gynecology, Chonnam National University Medical School

• Rhee J

  Korea Advanced Inst. Sci. And Technol. Taejon Kor

• Kim Seok

  Korea Food Research Institute Bundang Songnam

• RHEE Joon

  Department of Biological Science and Engineering, The Korea Advanced Institute of Science

• Kim Seok

  Department Of Molecular Science & Technology Ajou University

• Rhee Joon

  Department Of Biological Science And Engineering Korea Advanced Institute Of Science Of Technology

• Moon Kwang

  Agricultural Product Utilization Division Korea Food Research Institute

• Han D

  Korea Food Research Institute Bundang Songnam

• Han Daeseok

  Agricultural Product Utilization Division, Korea Food Research Institute

• Kim Seok

  Department Of Dermatology Seoul National University College Of Medicine

• Kim Seok

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• RHEE Joon

  Department of Biological Science & Engineering

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