Inhibition of DNA Duplication of Rheumatoid Synovial Fibroblast by N'-[β-D-Ribofuranosyl]-5-hydroxyimidazol-4-carboxamide, Mizoribine
スポンサーリンク
概要
- 論文の詳細を見る
The hyperplasia of synovial fibroblast plays critical roles in the propagation of rheumatoid synovitis. N'-[β-D-Ribofuranosyl]-5-hydroxyimidazol-4-carboxamide (mizoribine; MZR), an immunosuppressive agent, is reported to have beneficial effects in patients with rheumatoid arthritis (RA) for suppression of lymphocyte proliferation. In order to study the anti-rheumatic action of MZR, we have investigated its effect on DNA synthesis of RA synovial fibroblasts. DNA synthesis by synovial fibroblasts was evaluated by measuring the uptake of tritiated thymidine (^3H-TdR) after stimulation with interleukin-1β in the presence or absence of MZR. MZR suppressed ^3H-TdR incorporation by synovial fibroblasts in a concentration-dependent manner. This inhibition was significant with 1-2μg/ml MZR (p<0.01), the concentration attainable in sera of treated patients. MZR has been reported to inhibit inosine monophosphate (IMP) dehydrogenase, a key enzyme in the formation of guanine ribonucleotides from IMP. Therefore, to examine whether this suppression of cell proliferation by MZR is also due to the inhibition of IMP dehydrogenase, guanosine monophosphate (GMP), an end-product converted by IMP dehydrogenase, and MZR were added simultaneously to synovial fibroblast cultures. We found that 10μg/ml of GMP reversed the MZR-induced suppression of synovial fibroblast proliferation. These results suggest that MZR inhibits synovial fibroblast proliferation directly by depleting of intracellular guanine ribonucleotides, thereby possibly inhibiting rheumatoid synovitis by virtue of this action.
- 神戸大学の論文
- 2001-11-30
著者
-
Tomioka Masao
Department Of Orthopaedic Surgery Kobe University School Of Medicine
-
Hirata Soichiro
Faculty of Health Science, Kobe University School of Medicine
-
Ishikawa Hitoshi
Faculty of Health Science, Kobe University School of Medicine
-
Saura Ryuichi
Faculty of Health Science, Kobe University School of Medicine
-
Hirata Soichiro
Faculty Of Health Sciences Kobe University School Of Medicine
-
Hirata Soichiro
Faculty Of Health Science Kobe University School Of Medicine
-
Ishikawa Hitoshi
Faculty Of Health Sciences Kobe University School Of Medicine
-
Ishikawa Hitoshi
School Of Allied Medical Sciences Kobe University
-
Ishikawa Hitoshi
Faculty Of Health Science Kobe University School Of Medicine
-
Hirata Soichiro
Department Of Orthopedic Surgery Kobe University School Of Medicine
-
Saura R
Faculty Of Health Sciences Kobe University School Of Medicine
-
Saura Ryuichi
Faculty Of Health Science Kobe University School Of Medicine
-
Saura Ryuuichi
Faculty Of Health Science Kobe University School Of Medicine
-
Saura Ryuichi
Kobe University School Of Medicine
-
Hirata Souichirou
Faculty Of Health Science Kobe University School Of Medicine
-
Ishikawa Hitoshi
Faculty Of Health Science Kobe University
関連論文
- Lymphokine-Induced Synovitis : An Electron Microscopic Study
- Apoptosis of hypertrophic chondrocytes in rat cartilaginous growth plate
- Inhibiton of Thrombin-induced Cartilage Proteoglycan Release by Argatroban
- Osteochondritis of Hallux Sesamoid Bone : A Case Report
- Adenocarcinoma of the Prostate Presenting Initially as Intraspinal Tumors
- A Locked knee Due To A Large Nodule of Pigmented Villonodular Synovitis in Patello-Femoral Joint
- Posterior Interosseous Radial Nerve Neuropathy at the Arcade of Frohse
- Conversion of Fused Hip to Low-Friction Arthroplasty : A Case Report
- An Unusual Variant of Chronic Osteomyelitis in a Child
- Bilateral Hip Fractures in a Patient with Osteomalacia
- Avascular Necrosis of the Talus Following Apparently Minor Ankle injury : A Case Report
- Mycobacterial Osteomyelitis in Association with Infarction of Bone: Report of a Case
- Ulner Nerve Entrapment Neuropathy at the Cubital Tunnel Associated with Baseball Pitching During Adolescence
- Recurrent Lateral Subdislocation of the Tendon of the Long Biceps Brachii
- Pathokinesiological Discussion on Abduction Contracture of the Shoulder Joint Secondary to Partial Rupture of the Deltoid Muscle
- Avulsion Fractures of the Anterior Iliac Spines in Young Athletes
- Osteochondritis Dissecans of the Elbow Associated with Baseball During Adolescence
- Vascular Injury from Intrapelvic Migration of a Screw Used for the Intertrochanteric Fracture
- Bilateral Radial Nerve Palsies due to Sustained Physical Trauma
- Long-Term Follow-up of a Patient with Congenital Sensory Neuropathy
- Spacial Distribution of HLA-DR Positive Mononuclear Cells and Lymphocytes of Different Subsets in Rheumathoid Synovial Membrane
- B-Lymphocytes and HLA-DR Expressing Cells of Lymphocytic Infiltration in Rheumatoid Synovial Membrane
- Treatment of nonunion of the femoral neck fracture associated with avascular necrosis in a child
- Inter-relation between Variables of Physical Disability in Postoperative Patients with Adult Hip Disorders : A Statistical Approach
- Chronic Lymphatic Leukemia : Its Polymorphism of Monoclonal Surface Immunoglobulins
- Influence of Ankle Taping on Varus-valgus and Internal-external Rotation Movements of Knee Joint During Footwork Exercises
- Ambulatory Physical Activity is Associated with Walking Speed in Adult Women with Hip Disorders
- Low intensity pulsed ultrasound exposure increases prostaglandin E_2 release in human dermal fibroblasts
- Age- and Speed-Related Changes in Gait Parameters of Adult Women with Osteoarthritis of the Hip
- A Hip Pain Caused from Labral Tears Associated with Intraosseous Ganglion of Acetabulum in a Patient with Mild Hip Osteoarthrosis
- Nitric oxide synthesis of bovine articular chondrocytes induced by thrombin not through the thrombin receptor, protease-activated receptor-1 (PAR-1)
- Inhibition of DNA Duplication of Rheumatoid Synovial Fibroblast by N'-[β-D-Ribofuranosyl]-5-hydroxyimidazol-4-carboxamide, Mizoribine
- An immunohistochemical and immunoelectron microscopic study of beta integrins (α4β1, α5β1) and matrix metalloproteinases expression in rheumatoid synovial pannus formation in rheumatoid arthritis
- Aneurysmal Bone Cyst Arising From a Giant Cell Tumor of the Calcaneus
- Beta Integrins (α4β1, α5β1) and Matrix Metalloproteinases Expression in Synovial Pannus Formation in Rheumatoid Arthritis
- Effect of Prostaglandin E_2 and EP Receptor Agonists on Cell Proliferation and Basic Fibroblast Growth Factor Synthesis of Rheumatoid Synovial Fibroblasts in Vitro
- A Rheumatoid Nodule on the Extensor Tendon of the Wrist
- Arthritis Caused by Chlamydia in Reiter Syndrome : A Case Report of Long-term Follow Up
- Effect of Cyclooxygenase Inhibitor on Rheumatoid Synovial Fibroblasts Proliferation in Vitro
- Different Myoelectric Behavior of the Erector Spinae in Flexion and Reextension During Trunk Foward Bending
- Multicentric Reticulohistiocytosis : A Case Report of Twenty Years Follow Up
- The Effect of Activities of Daily Living (ADL) Instructions to Rheumatoid Arthritis Patients
- Lumbopelvic Movemement Interaction During Trunk Forward Bending in Healthy Young Adults
- Interaction of VLA-5 Molecule With Rheumatoid Articular Cartilage Surface : An Electron Microscopic Evidence of Expression of VLA-5 on Pannus Invading Cells
- Chronic Osteomyelitis of the Proximal Femur in SAPHO Syndrome with Avascular Necrosis of the Femoral Head : A Case Report
- Deep venous thrombosis in patients with acute cervical spinal cord injury in a Japanese population : assessment with Doppler ultrasonography
- The Changes of Intramuscular Oxygen Tension by Local Cold Application in the Rat
- The Role of Adhesion Molecules In Synovial Pannus Formation
- Role of Adhesion Molecules in the Lymphoid Cell Distribution in Rheumatoid Synovial Membrane
- Surgical Treatment of Painful Bipartite Patella Associated with Young Athletes
- An Epidemiological Study on Low Back Pain in Fishermen Working in Seaweed Farming
- Effects of Heat on Intraosseous Blood Flow
- Home Stretching Exercise is Effective for Improving Knee Range of Motion and Gait in Patients with Knee Osteoarthritis
- Myositis Ossificans in a Young Gymnast
- A Simple Reconstructive Technique of Rupture of the Long Head Tendon of the Biceps Brachii
- Effect of Hyaluronic Acid on Proteoglycan Metabolism in Cultured Bovine Articular Chondrocytes
- Adult Onset Juvenile Rheumatoid Arthritis : Febrile Episodes Controllable at Out-Patient Clinic
- An Effect of Natural Interferon-α on A Myeloma Patient Suffering from Severe Low Back Pain and Hypercalcemia
- Complement Deficiency State associated with Generalized Skin Eruptions in a SLE Patient
- Property of Proximal Tibia in the Rat in Determination of Intraosseous Blood Flow
- A Study of the Enhancing Factors Affecting the Incidence of Hip Dislocation and Scoliosis in Children with Cerebral Palsy
- Low Dose Continuous Chemotherappy on Myeloma Patients - A Comparison between CP and MP
- Continuous Low-dose vs Intermittent High-dose Schedules of Mephalan-Prednisolone in Myeloma
- Plasma Cell Dyscrasia Occuring during the Clinical Course of Sjogren's Syndrome
- T Cell Increase in the Peripheral Blood and some Antitumor Effect by Streptococcal Agent
- Receptor-Ligand Interaction of Adhesion Molecules Occurring in the Rheumatoid Synovial-Pannus Junction
- Cytokine Enhancements of Monocyte Attachment on the Surface of Cartilage: A Possible Trigerring Factors of Pannus Formation in Arthritis
- A Mechanism of Cisplatin Action : Antineoplastic Effect through Inhibition of Neovascularization
- Total Hip Arthroplasty with Chiari Pelvic Osteotomy in Dysplastic Hips
- Acetabular Reconstruction Using Protrusio Acetabli (PA) Socket in Revision Total Hip Arthroplasty
- Bipolar Hip Arthroplasty for Osteoarthritis of the Hip Joint
- Rheumatoid pannus formation: synovial cell attachment to the surface of cartilage
- Expression of Adhesion Molecules in the Lymphoid Cell Distribution in Rheumatoid Synovial Membrane
- An Immunohistochemical and Immunoelectron Microscopic Study of Adhesion Molecules in Synovial Pannus Formation in Rheumatoid arthritis
- β1-Integrin Expression in the Rheumatoid Synovial-Pannus Formation
- The antiproliferative effect of mizoribine on rheumatoid synovial fibroblast mediated by induction of apoptosis
- Does High Dose Methylprednisolone Sodium Succinate Really Improve Neurological Status in Patient With Acute Cervical Cord Injury? : A Prospective Study About Neurological Recovery and Early Complications
- Effect of prostsglandin e_2 on nitric oxide synthesis in articular chondrocytes
- Posterior Release Surgery for Severe Flexion Contracture of the knee in Patients with Rheumatoid Arthritis
- Ultrastructural Investigations of Calcium Pyrophosphate Dihydrate Crystal Depositions in Chondrocalcinosis
- Effect of Substance-P on Metalloproteinase Release and Extracellular Matrix Accumulation of Articular Chondrocytes
- Risk Factors for Lumbosacral Plexus Palsy Related to Pelvic Fracture
- The Role of Cyclooxygenase-2 in Lumbar Disc Herniation
- Intervertebral disc cell apoptosis by nitric oxide : biological understanding of intervertebral disc degeneration
- Skeletal Muscle Pseudohypertrophy in AL Amyloidosis
- Posterior Interosseous Nerve Syndrome in Baseball Pitcher - A Case Report
- Possible Roles lnterleukin-1 and Collagenase in the Pannus Formation in Rheumatoid Arthritis
- Magnetic Resonance Imaging of Syringomyelia
- Extramedullary Plasmacytoma of the Lymph Nodes
- Ultrastructural Investigations of Polymyositis Muscle in a Patient with Rheumatoid Arthritis
- Primary Acquired Hypogammaglobulinemia Associated With Behcet Disease - An Observation For 12 Years
- Spina Ventosa: Multiple Tuberculous Dactylitis : A Case Report
- Avascular Necrosis of the Femoral Head with Diffuse Bone Marrow Edema Pattern on MR imaging : A Case Report
- Effects of selective-wavelength block filters on pupillary light reflex under red and blue light stimuli
- Factor analysis on oral health care for acute hospitalized patients in Japan
- Developing role of dental hygienists in Japan