The antiproliferative effect of mizoribine on rheumatoid synovial fibroblast mediated by induction of apoptosis
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概要
- 論文の詳細を見る
In order to investigate the mechanism of anti-rheumatic action ofmizoribine (MZR), antiproliferative effect of MZR on synovial fibroblasts obtainedfrom rheumatoid arthritis (RA) patients were examined. To examine the effect ofMZR on DNA synthesis, total radioactivity of 3H-thymidine (3H-TdR) incorporatedinto the synovial fibroblasts was measured. Also quantification of DNA fragmentationof synovial fibroblasts in the cultured supernatant and cell associated Bcl-2 protein,which is suspected of interfering with apoptosis, were performed with enzyme-linkedimmunosorbent assay.MZR suppressed 3H-TdR incorporation into synovial fibroblasts in a dosedependent fashion. Significant inhibition (P<0.01) was attained at the concentration ofmore than 1 µg/ml of MZR. However, induction of DNA fragmentation which ischaracteristic of apoptosis, were observed at only 10 µg/ml of MZR over 72h-incubation significantly. In terms of the Bcl-2 expression of synovial fibroblasts, upto 10 µg/ml of MZR has no effect on the expression of this protooncogene bcl-2expression. These results suggest that MZR might suppress the growth of rheumatoidpannus by inhibition of synovial fibroblast proliferation partially through theinduction of apoptosis of synovial fibroblast without modulating Bcl-2 expression.
- 神戸大学の論文
著者
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Nakagami K
Institute For Life Science Research Asahi Chemical Industry Co. Ltd.
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Nakagami Keiji
Institute For Life Science Research Asahi Chemical Industry Co. Ltd
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MIZUNO Kosaku
Department of Orthopaedic Surgery, Kobe University School of Medicine
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Saura Ryuichi
Department of Orthopaedic Surgery, Kobe University School of Medicine
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Mizuno K
Department Of Orthopedic Surgery Kobe University Graduate School Of Medicine
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Mizuno Kosaku
Department Of Orthopaedic Surgery Kobe University School Of Medicine
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Saura R
Faculty Of Health Sciences Kobe University School Of Medicine
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Saura Ryuichi
Department Of Orthopaedic Surgery Kobe University School Of Medicine
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Saura Ryuuichi
Faculty Of Health Science Kobe University School Of Medicine
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Imaizumi Yasuhiko
Department of Orthopaedic Surgery, Kobe University School of Medicine
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Saura Ryuichi
Kobe University School Of Medicine
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Imaizumi Yasuhiko
Department Of Orthopaedic Surgery Kobe University School Of Medicine
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Imaizumi Yasuhiko
Faculty Of Health Sciences Kobe University School Of Medicine
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Nakagami Keiji
Department of Orthopaedic Surgery, Kobe University School of Medicine
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Saura Ryuichi
Institute for Life Science Research, Asahi Chemical Industry Co. Ltd.
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