Effect of prostsglandin e_2 on nitric oxide synthesis in articular chondrocytes
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概要
- 論文の詳細を見る
Both nitric oxide (NO) and prostaglandin E2 (PGE2) are known to play an important role in cartilage metabolism. The present study investigated the novel intercellular mechanism of inducible NO synthase (iNOS) induction mediated by PGE2 in articular chondrocytes. Bovine articular chondrocytes were stimulated by cyclic adenosine monophosphate (cAMP) elevating agents like PGE2 in the presence of interleukin- Ib (IL-Ib). NO generation was measured by using the Griess reaction. Inducible NOSmRNA was semi-quantitated by reverse transcription-polymerase chain reaction (RT PCR). While little NO was released from articular chondrocytes in the presence of PGE2 or direct adenylate cyclase activator such as forskolin, synergistic augmentation of NO generation was observed when chondrocytes were stimulated by PGE2 or forskolin in combination with IL-I?. Further expression of iNOS mRNA by stimulation of PGE2 in the presence of IL-Ib simultaneously was also detected by RT-PCR in comparison with the mRNA induction by IL-Ib stimulation alone. These results indicated that PGE2 might modulate the articular cartilage metabolism by augmentation of chondrocyte NO synthesis in inflammatory process through cAMP-protein kinase A system.
- 神戸大学の論文
著者
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Itoh Hiroshi
神戸大学 整形外科
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Itoh Hiroshi
神戸大学医学系研究科
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Saura Ryuichi
神戸大学医学部
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Mizuno Kosaku
神戸大学医学部
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Saura R
Faculty Of Health Sciences Kobe University School Of Medicine
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Saura Ryuuichi
Faculty Of Health Science Kobe University School Of Medicine
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Onga Takafumi
神戸大学医学部
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