A Specific and Sensitive Assay for Gefitinib Using the Enzyme-Linked Immunosorbent Assay in Human Serum(Biochemistry)
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概要
- 論文の詳細を見る
The epidermal growth factor tyrosine kinase inhibitor gefitinib is a novel, molecularly targeted agent that has been approved for the treatment of advanced non-small cell lung cancer. This paper reports a specific and sensitive enzyme-linked immunosorbent assay (ELISA) for pharmacokinetic studies of gefitinib. Anti-gefitinib antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using diazotized 4-amino-2-methoxy-1-[3-(morpholin-4-yl)propoxy]benzene. Enzyme labeling of gefitinib with β-D-galactosidase was similarly performed using a diazotized 4-amino-2-methoxy-1-[3-(morpholin-4-yl)propoxy]benzene. A simple ELISA for gefitinib was developed using the principle of direct competition between gefitinib and the enzyme marker for anti-gefitinib antibody which had been adsorbed to the plastic surface of a microtiter plate. Gefitinib concentrations in serum lower than 800pg/ml were measurable reproducibly using the ELISA. Crossreactivity data showed that the antibody well recognizes both the 3-morpholinopropoxy and methoxy moieties well, and thus is sufficiently specific for the structure of gefitinib. Using this assay, drug levels were easily measured in the serum of rabbits after oral administration of gefitinib at a single dose of 5mg/kg. The specificity and sensitivity of the ELISA for gefitinib should provide a valuable new tool for use in pharmacokinetic and toxicity studies of gefitinib.
- 公益社団法人日本薬学会の論文
- 2005-10-01
著者
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Mori Masao
Faculty Of Hospital Pharmacy Saga Medical School
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MORI Masato
Department of Pediatrics, Jichi Medical University
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Mori M
Department Of Pharmacy Saga University Hospital
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Mori Masato
Department Of Pediatrics Jichi Medical University
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Mori M
Faculty Of Hospital Pharmacy Saga Medical School
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Mori M
The Institute Of Physical And Chemical Research (riken)
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Saita T
Department Of Pharmacy Saga University Hospital
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Fujito H
Department Of Pharmacy Saga University Hospital
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森 昌斗
Hospital Pharmacy Saga Medical School
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SAITA Tetsuya
Department of Pharmacy, Saga University Hospital
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FUJITO Hiroshi
Department of Pharmacy, Saga University Hospital
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Mori Masato
Department Of Biological Science Faculty Of Life And Environmental Science Shimane University
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