Establishment of an Enzyme-Linked Immunosorbent Assay for Measurement of Sotalol(Analytical Biochemistry)

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概要

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• We have established an enzyme-linked immunosorbent assay suitable for routine monitoring of serum levels of sotalol. Anti-sotalol antibody was obtained by immunizing rabbits with sotalol conjugated with bovine serum albumin using the N-succinimidyl ester method. An enzyme marker was similarly prepared by coupling sotalol with β-D-galactosidase. The detection limit of sotalol by the enzyme-linked immunosorbent assay was approximately 32 ng/ml with 50-μl samples. This assay was specific for sotalol because of very slight cross-reactivity with 4-(methanesulfonylamino)benzonitrile (1.6%), but none with D,L-isoproterenol. Using this assay, drug levels were easily measured in the serum of rabbits after oral administration of sotalol at a single dose of 3 mg/kg. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of sotalol.

• 公益社団法人日本薬学会の論文
• 2004-01-01

著者

• Mori Masao

  Faculty Of Hospital Pharmacy Saga Medical School

• Mori M

  Department Of Pharmacy Saga University Hospital

• Mori M

  Faculty Of Hospital Pharmacy Saga Medical School

• Mori M

  The Institute Of Physical And Chemical Research (riken)

• NAKANO Yukitaka

  Faculty of Hospital Pharmacy, Saga Medical School

• MORI Masato

  Faculty of Hospital Pharmacy, Saga Medical School

• SAITA Tetsuya

  Faculty of Hospital Pharmacy

• FUJITO Hiroshi

  Faculty of Hospital Pharmacy Saga Medical School

• Saita T

  Department Of Pharmacy Saga University Hospital

• Fujito H

  Department Of Pharmacy Saga University Hospital

• 森 昌斗

  Hospital Pharmacy Saga Medical School

• Nakano Yukitaka

  Faculty Of Hospital Pharmacy Saga Medical School

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