Development of an Enzyme-Linked Immunosorbent Assay for the Quantification of Amiodarone

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概要

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• A sensitive and specific enzyme-linked immunosorbent assay for an antiarrhythmic drug, amiodarone (AMI), was developed, which is capable of measuring levels as low as 16 ng/ml. Anti-AMI antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. Enzyme labeling of AMI with β-D-galactosidase was similarly performed using a diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. This enzyme-linked immunosorbent assay was specific for AMI and showed a very slight cross-reactivity (1.25%) with its major metabolite, mono-N-desethylamiodarone. The values of the AMI concentrations measured by this assay were in good correlation to those by HPLC. Its analytical applicability was demonstrated by a kinetic study with human liver microsomes. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of AMI.

• 公益社団法人日本薬学会の論文
• 2002-08-01

著者

• Mori Masao

  Faculty Of Hospital Pharmacy Saga Medical School

• Mori M

  Department Of Pharmacy Saga University Hospital

• Mori M

  Faculty Of Hospital Pharmacy Saga Medical School

• Mori M

  The Institute Of Physical And Chemical Research (riken)

• MORI Masato

  Faculty of Hospital Pharmacy, Saga Medical School

• SAITA Tetsuya

  Faculty of Hospital Pharmacy

• FUJITO Hiroshi

  Faculty of Hospital Pharmacy Saga Medical School

• Saita T

  Department Of Pharmacy Saga University Hospital

• Fujito H

  Department Of Pharmacy Saga University Hospital

• 森 昌斗

  Hospital Pharmacy Saga Medical School

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