Determination of Topotecan by ELISA
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概要
- 論文の詳細を見る
A highly sensitive ELISA for the determination of (s)-9-dimethylaminomethyl-10-hydroxy-camptothecin(topotecan)capable of measuring as low as 80 pg/ml was developed. Anti-topotecan antibody was obtained by immunizing rabbits with topotecan conjugated with bovine serum albumin using diazotized m-aminobenzoic acid as a cross-linker. Enzyme labeling of topotecan with β-D-galactosidase was performed by utilizing another cross-linker, N-(4-diazophenyl)maleimide. The specificity of this ELISA appears to be primarily toward the lactone moiety of topotecan, showing a very slight cross-reactivity with the lactone opened-ring of topotecan. The values for the topotecan concentrations detected using this assay were comparable with those detected using HPLC. There was a good correlation between the values determined by the two methods. These findings suggest that this ELISA can detect the natural amounts of the lactone form. Using this assay, drug levels were easily determined in the blood and urine of rats for 5h after i.v.administration of topotecan at a single dose of 1 mg/kg. The sensitivity and specificity of the ELISA should provide a useful tool for developing pharmacokinetic and pharmacodynamic studies of topotecan.
- 公益社団法人日本薬学会の論文
- 2001-04-01
著者
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Mori Masao
Faculty Of Hospital Pharmacy Saga Medical School
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Mori M
Department Of Pharmacy Saga University Hospital
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Mori M
Faculty Of Hospital Pharmacy Saga Medical School
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Mori M
The Institute Of Physical And Chemical Research (riken)
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MORI Masato
Faculty of Hospital Pharmacy, Saga Medical School
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SAITA Tetsuya
Faculty of Hospital Pharmacy
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FUJITO Hiroshi
Faculty of Hospital Pharmacy Saga Medical School
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Saita T
Department Of Pharmacy Saga University Hospital
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Fujito H
Department Of Pharmacy Saga University Hospital
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森 昌斗
Hospital Pharmacy Saga Medical School
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