Screening of Furanocoumarin Derivatives in Citrus Fruits by Enzyme-Linked Immunosorbent Assay(Analytical Biochemistry)
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概要
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This paper reports a sensitive and specific enzyme-linked immunosorbent assay for screening of furanocoumarin derivatives as cytochrome P450 3A4 inhibitors in citrus fruits. Anti-6',7'-dihydroxybergamottin antibody was obtained by immunizing rabbits with 6',7'-dihydroxybergamottin conjugated with bovine serum albumin using the N-succinimidyl ester method. An enzyme marker was similarly prepared by coupling 6',7'-dihydroxybergamottin with β-D-galactosidase. The enzyme-linked immunosorbent assay is capable of detecting as little as 800 pg/ml of 6',7'-dihydroxybergamottin and 4 ng/ml of bergamottin. Cross-reactivity data showed that the antibody well recognizes both the furanocoumarin and 6,7-dihydroxy-3,7-dimethyloct-2-enyloxy moieties of the 6',7'-dihydroxybergamottin, and is thus specific to the structure of furanocoumarin derivatives containing geranyloxy side chain as the cytochrome P450 3A4 inhibitors in grapefruit juice. The antibody was, therefore, used for screening a large number of citrus fruits for furanocoumarin derivatives such as 6',7'-dihydroxybergamottin. Fifteen citrus fruits were examined and significant reactivity was observed in 8 of these: red pummelo, sweetie, melogold, banpeiyu pummelo, hassaku, sour orange, lime and natsudaidai. This enzyme-linked immunosorbent assay may be a powerful tool for screening for furanocoumarin derivatives as cytochrome P450 3A4 inhibitors in grapefruit juice.
- 公益社団法人日本薬学会の論文
- 2004-07-01
著者
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Mori Masao
Faculty Of Hospital Pharmacy Saga Medical School
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MORI Masato
Department of Pediatrics, Jichi Medical University
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Mori M
Department Of Pharmacy Saga University Hospital
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Mori Masato
Department Of Pediatrics Jichi Medical University
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Mori M
Faculty Of Hospital Pharmacy Saga Medical School
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Mori M
The Institute Of Physical And Chemical Research (riken)
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Saita T
Department Of Pharmacy Saga University Hospital
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Fujito H
Department Of Pharmacy Saga University Hospital
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森 昌斗
Hospital Pharmacy Saga Medical School
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SAITA Tetsuya
Department of Pharmacy, Saga University Hospital
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FUJITO Hiroshi
Department of Pharmacy, Saga University Hospital
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Mori Masato
Department Of Biological Science Faculty Of Life And Environmental Science Shimane University
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