Development of ELISAs for Irinotecan and Its Active Metabolite SN-38
スポンサーリンク
概要
- 論文の詳細を見る
Two highly sensitive and specific enzyme-linked immunosorbent assays (ELISAs) for the determination of 7-thyl-10-[4-(1-piperidino)-1-piperidino]carbonyloxy-camptothecin (irinotecan) and 7-ethyl-10-hydroxycamp-tothecin (SN-38), an active metabolite of irinotecan, were developed, which are capable of measuring as low as 16 and 160 pg of each drug/ml, respectively.Anti-irinotecan antibody was obtained by immunizing rabbits with irinotecan conjugated with mercapto-succinyl bovine serum albumin (MS.BSA) using N-(4-diazophenyl)maleimide (DPM) as a heterobifunctional coupling agent. An enzyme marker was similarly prepared by coupling irinotecan with horseradish peroxidase (HRP) via DPM. This ELISA for irinotecan was specific for irinotecan and showed almost no cross-reactivity with its active metabolite SN-38. Anti-SN-38 antibody was obtained by immunizing rabbits with SN-38 conjugated with BSA using the N-succinimidyl ester method. An enzyme marker was prepared by coupling SN-38 with HRP employing DPM. The ELISA for SN-38 was specific to SN-38 and showed a very slight cross-reactivity with irinotecan (0.08%). Using the 2 assays, we reconfirmed the rapid metabolite of irinotecan with rat serum. The 2 ELISAs may be a valuable tool for studies of the pharmacokinetics and pharmacodynamics of these drugs.
- 公益社団法人日本薬学会の論文
- 2000-08-01
著者
-
Mori Masao
Faculty Of Hospital Pharmacy Saga Medical School
-
Mori M
Department Of Pharmacy Saga University Hospital
-
Mori M
Faculty Of Hospital Pharmacy Saga Medical School
-
Mori M
The Institute Of Physical And Chemical Research (riken)
-
MORI Masato
Faculty of Hospital Pharmacy, Saga Medical School
-
SAITA Tetsuya
Faculty of Hospital Pharmacy
-
FUJITO Hiroshi
Faculty of Hospital Pharmacy Saga Medical School
-
Saita T
Department Of Pharmacy Saga University Hospital
-
Fujito H
Department Of Pharmacy Saga University Hospital
-
森 昌斗
Hospital Pharmacy Saga Medical School
関連論文
- A Highly Sensitive Enzyme-Linked Immunosorbent Assay (ELISA) for Antitumor Polyacetylenic Alcohol, Panaxytriol
- The First Specific Antibody against Cytotoxic Polyacetylenic Alcohol, Panaxynol
- Inhibitory Effect of cis-Dehydromatricaria Ester Isolated from Solidago altissima on the Growth of Mammalian Cells
- Cytotoxic Activity of Polyacetylene Compounds in Panax ginseng C. A. MEYER
- Studies on the Panaxytriol of Panax ginseng C. A. MEYER. Isolation, Determination and Antitumor Activity
- Antiproliferative Constituents from Umbelliferae Plants. V. A New Furanocoumarin and Falcarindiol Furanocoumarin Ethers from the Root of Angelica japonica
- Antiproliferative Constituents in Umbelliferae Plants. IV. Constituents in the Fruits of Anthriscus sylvestris HOFFM.
- Antiproliferative Constituents in Umbelliferae Plants. III. Constituents in the Root and the Ground Part of Anthriscus sylvestris HOFFM.
- Antiproliferative Constituents in Umbelliferae Plants II. : Screening for Polyacetylenes in Some Umbelliferae Plants, and Isolation of Panaxynol and Falcarindiol from the Root of Heracleum moellendorffii
- Structures and Antiproliferative Activity of Saponins from Sechium pittieri and S. talamancense
- Studies on the Synthesis of Pyridazine Derivatives. XIII. Synthesis of Imidazo-[4,5-d]- and v-Triazolo[4,5-d]pyridazine Derivatives and Reaction of 4-Alkoxy-v-triazolo[4,5-d]pyridazines with Various Amines
- A Highly Sensitive ELISA for the Quantification of Polymyxin B Sulfate in Human Serum
- TRANSIENT BEHAVIOUR OF THE MEAN WAITING TIME AND ITS EXACT FORMS IN M/M/1 AND M/D/1
- Stereoselective Total Synthesis of Tri- and Tetrahexoside Wheat Flour Ceramide
- Stereoselective Total Synthesis of Wheat Flour Ceramide Dihexoside(Organic Chemistry)
- synthetic Studies of an 18-Membered Antitumor Macrolide, Tedanolide.5. Stereoselective Synthesis of the C13-C23 Part via Condensation of Two Fragments, C13-C17 and C18-C21,by Taking Adventage of the 3,4-Dimethoxybenzyl Protecting Group
- Synthetic Studies of the 18-Membered Antitumor Macrolide, Tedanolide. 3. Stereocontrolled Synthesis of the C1-C12 Part via a Synthesis of the C1-C7 Fragment by a Mismatched but Efficient Sharpless Dihydroxylation and Its Coupling with the C8-C11 Fragment
- Synthetic Studies of 18-Membered Antitumor Macrolide, Tedanolide. 2. Stereoselective Synthesis of the C1-C7 Fragment via a Mismatched but Highly Efficient Sharpless Dihydroxylation
- Total Synthesis of Prostaglandin F_ Using Nickel-Catalyzed Stereoselective Cyclization of 1,3-Diene and Tethered Aldehyde via Transmetalation of Nickelacycle with Diisobutylaluminum Acetylacetonate
- 1999年度日本味と匂学会第33回大会
- A Specific and Sensitive Assay for Gefitinib Using the Enzyme-Linked Immunosorbent Assay in Human Serum(Biochemistry)
- Screening of Furanocoumarin Derivatives in Citrus Fruits by Enzyme-Linked Immunosorbent Assay(Analytical Biochemistry)
- Establishment of an Enzyme-Linked Immunosorbent Assay for Measurement of Sotalol(Analytical Biochemistry)
- Development of Enzyme-Linked Immunosorbent Assay for Therapeutic Drug Monitoring of Mexiletine
- Development of an Enzyme-Linked Immunosorbent Assay for the Quantification of Amiodarone
- ELISA for the Quantification of Pilsicainide
- Determination of Topotecan by ELISA
- Development of ELISAs for Irinotecan and Its Active Metabolite SN-38
- Release Rates of Kampo Extract from Witepsol Suppositories and Its Rectal Absorption in Rabbits
- Nickel-Promoted Carboxylation/Cyclization Cascade of Allenyl Aldehyde under an Atmosphere of CO2