Development of Enzyme-Linked Immunosorbent Assay for Therapeutic Drug Monitoring of Mexiletine
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概要
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This paper reports a sensitive and specific enzyme-linked immunosorbent assay for determination of the antiarrhythmic drug mexiletine in human serum. Anti-mexiletine antibody was obtained by immunizing rabbits with an antigen conjugated with mercaptosuccinyl bovine serum albumin using N-(ε-maleimidocaproyloxy)succinimide as a heterobifunotional coupling agent. Enzyme labeling of mexiietine with β-D-galactosidase was performed using glutaraldehyde. In this assay, the mexiletine to be quantified is chemically modified by acetic anhydride allowed to compete with a mexiletine-β-D-galactosidase conjugate for binding to a limited amount of an anti-mexiletine antibody which was used to coat the wells of a microtiter plate. Mexiletine concentrations lower than 80 ng/ml were measurable reproducibly by the enzyme-linked immunosorbent assay. This assay was specific for mexiletine and showed very slight cross-reactivity with its major metabolite, 2-hydroxymethylmexiletine (1.5%), but none with p-hydroxymexiletine. The values of serum mexiletine levels from 15 patients by this enzyme-linked immunosorbent assay were comparable with those measured by HPLC. There was a good correlation between the values determined by the two methods. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of mexiletine.
- 公益社団法人日本薬学会の論文
- 2003-06-01
著者
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Mori Masao
Faculty Of Hospital Pharmacy Saga Medical School
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Mori M
Department Of Pharmacy Saga University Hospital
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Mori M
Faculty Of Hospital Pharmacy Saga Medical School
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Mori M
The Institute Of Physical And Chemical Research (riken)
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MORI Masato
Faculty of Hospital Pharmacy, Saga Medical School
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SAITA Tetsuya
Faculty of Hospital Pharmacy
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FUJITO Hiroshi
Faculty of Hospital Pharmacy Saga Medical School
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Saita T
Department Of Pharmacy Saga University Hospital
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Fujito H
Department Of Pharmacy Saga University Hospital
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森 昌斗
Hospital Pharmacy Saga Medical School
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