A Modified Fluorescence Polarization Immunoassay Method Incorporating Fat Emulsion (FE-FPIA) to Determine Cyclosporin A Concentrations in Rat Skin
スポンサーリンク
概要
- 論文の詳細を見る
We have developed a simple, sensitive and reliable assay procedure for cyclosporin A (CyA), a modified fluorescence polarization immunoasay method incorporating fat emulsion (FE-FPIA), to determine the CyA content in rat skin. The conventional fluorescence polarization immunoassay (FPIA) method for CyA using a commercially available FPIA kit, TDX^[○!R] cyclosporine monoclonal whole blood, was modified. A fat emulsion for intravenous infusion, Intralips^[○!R], was incorporated for dissolving the CyA extracted from the skin tissue, and a mixture of MeOH/purified water was used as the sample pretreatment medium instead of the precipitation reagent in the conventional FPIA kit intended for whole blood samples. These modifications enabled us to produce a reliable and the sensitive assay of CyA in skin tissue. The reproducibility (coefficient of variation), detection limit, and assay time for FE-FPIA were below 2%, 25 ng/ml, and about 24 min/24 samples, respectively, and were comparable with those for the whole blood samples determined by the conventional FPIA. Pre-purification of samples required by the HPLC assay is not needed in the FE-FPIA method. The usefulness of the FE-FPIA method in evaluating the topical pharmacokinetics of CyA in skin is discussed.
- 公益社団法人日本薬学会の論文
- 1999-05-15
著者
-
NAKAMURA Toshiaki
Department of Anesthesiology, Nagasaki Rosai Hospital
-
Kawasaki Hiromu
Department Of Clinical Pharmaceutical Science Graduate School Of Pharmaceutical Sciences Okayama Uni
-
Masada Mikio
Department Of Hospital Pharmacy Fukui Medical University
-
Kurosaki Yuji
Department Of Clinical Pharmaceutical Science Graduate School Of Pharmaceutical Sciences Okayama Uni
-
Kawasaki Hiromu
Department Of Clinical And Pharmaceutical Science Graduate School Of Medicine Dentistry And Pharmace
-
Kurosaki Yuji
Department Of Clinical Pharmaceutical Science Faculty Of Pharmaceutical Sciences Graduate School Of
-
ASADA Tomoya
Department of Clinical Pharmaceutical Science, Graduate School of Pharmaceutical Sciences, Okayama U
-
YASUMOTO Nanae
Department of Clinical Pharmaceutical Science, Graduate School of Pharmaceutical Sciences, Okayama U
-
Asada Tomoya
Department Of Clinical Pharmaceutical Science Graduate School Of Pharmaceutical Sciences Okayama Uni
-
Yasumoto Nanae
Department Of Clinical Pharmaceutical Science Graduate School Of Pharmaceutical Sciences Okayama Uni
-
Nakamura Toshiaki
Department Of Anesthesia Nagasaki Rosai Hospital
-
Nakamura Toshiaki
Department Of Hospital Pharmacy Fukui Medical University
関連論文
- Royal Jelly Ameliorates Insulin Resistance in Fructose-Drinking Rats(Pharmacognosy)
- Close correlation of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate, an intracellular active metabolite, to the therapeutic efficacy of N(4)-behenoyl-1-beta-D-arabinofuranosylcytosine therapy for acute myelogenous leukemia.
- Monitoring of intracellular 1-beta-D-arabinofuranosylcytosine 5'-triphosphate in 1-beta-D-arabinofuranosylcytosine therapy at low and conventional doses.
- Comparism of Hemodynamic and Catecholamine Responses between Propofol and Thiamylal Used in a Rapid Sequence Induction
- Effect of Aripiprazole on 5-HT_2 Receptor-mediated Wet-Dog Shake Responses and Disruption of Prepulse Inhibition in Rats
- An Approach to Predict the Ductus-Arteriosus Dilating Effect Induced by Lipo-Prostaglandin E_1 in Newborn Rats Lacking Plasma Concentration-Time Data by the Pharmacological Response Kinetic Model
- Chronic Treatment with Imipramine and Lithium Increases Cell Proliferation in the Hippocampus in Adrenocorticotropic Hormone-Treated Rats
- Effect of Methamphetamine and Imipramine on Cerebral Ischemia-Induced Hyperactivity in Mongolian Gerbils
- A case of contact dermatitis due to Curcuma longa (turmeric)
- Counseling of Patients with Bronchial Asthma, and Clinical Pharmacy Practice and Education of Pharmacy School Students
- Malfunction of Vascular Control in Lifestyle-Related Diseases : Distribution of Adrenomedullin-Containing Perivascular Nerves and Its Alteration in Hypertension
- 3-D FDTD Analysis of Sound Field Focused by Biconcave Acoustic Lens for Normal Incidence
- Consideration of Radiation Impedance Calculation Methods for Cylindrical Array Sound Source
- Neurotrophic Effect of Hepatic Growth Factor (HGF) on Reinnervation of Perivascular Calcitonin Gene-Related Peptide (CGRP)-Containing Nerves Following Phenol-Induced Nerve Injury in the Rat Mesenteric Artery
- Increased DOI-Induced Wet-Dog Shakes in Adrenocorticotropic Hormone-Treated Rats Are Not Affected by Chronic Imipramine Treatment : Possible Involvement of Enhanced 5-HT_-Receptor Expression in the Frontal Cortex
- Influence of Lactation on Plasma Phenobarbital Concentrations in Rats
- Simple and rapid analysis of lamotrigine, a novel antiepileptic, in human serum by high-performance liquid chromatography using a solid-phase extraction technique
- Cellular Basis of Induction of Contact Hypersensitivity and Tolerance
- Estimation of Correlation of Acoustic Reciprocal Transmissions Using Central Equatorial Pacific Tomography Data
- Estimation of the Travel Time Using the New Measurement Technique for Long-range Transmission
- Estimation of the Stability of Acoustic Reciprocal Transmission in Long-Range Propagation at the Central Equatorial Pacific
- Selegilin Exerts Antidepressant-Like Effects During the Forced Swim Test in Adrenocorticotropic Hormone-Treated Rats
- Effects of Imipramine and Bupropion on the Duration of Immobility of ACTH-Treated Rats in the Forced Swim Test : Involvement of the Expression of 5-HT_ Receptor mRNA(Pharmacology)
- Effects of Long-Term Treatment With Calcium Antagonists on Periarterial Nerve Function in the Mesenteric Artery of Spontaneously Hyupertensive Rats
- Long-Term Treatment With Angiotensin Converting Enzyme Inhibitor Restores Reduced Calcitonin Gene-Related Peptide-Containing Vasodilator Nerve Function in Mesenteric Artery of Spontaneously Hypertensive Rats
- CHANGES IN CIRCADIAN RHYTHM OF LOCOMOTOR ACTIVITY INDUCED BY CEREBRAL ISCHEMIA IN MONGOLIAN GERBILS
- Pharmacokinetic analysis of flomoxef in children undergoing cardiopulmonary bypass and modified ultrafiltration
- Hemodynamic and catecholamine responses during rapid sequence induction with propofol in hypertensive patients
- Effect of Heat Exposure on Aminophylline-Induced Convulsions in Mice
- ENDOTHELIUM-INDEPENDENT VASORELAXATION INDUCED BY ACETYLCHOLINE
- Malfunction of Vascular Control in Lifestyle-Related Diseases : Preface
- Altered function of nitrergic nerves inhibiting sympathetic neurotransmission in mesenteric vascular beds of renovascular hypertensive rats
- Does Drug-induced Hypersensitivity Syndrome Elicit Bullous Pemphigoid?
- Acute Hyperglycemia and Hyperinsulinemia Enhance Adrenergic Vasoconstriction and Decrease Calcitonin Gene-Related Peptide-Containing Nerve-Mediated Vasodilation in Pithed Rats
- Chronic Hyperinsulinemia Enhances Adrenergic Vasoconstriction and Decreases Calcitonin Gene-Related Peptide-Containing Nerve-Mediated Vasodilation in Pithed Rats
- Chronic Angiotensin II Inhibition Increases Levels of Calcitonin Gene-Related Peptide mRNA of the Dorsal ~Root Ganglia in Spontaneously Hypertensive Rats
- Age-Related Decrease in Calcitonin Gene-Related Peptide mRNA in the Dorsal Root Ganglia of Spontaneously Hypertensive Rats
- Effects of Recombinant Human Growth/Differentiation Factor-5 Using in vitro and in vivo Experiments
- Sound Pressure Fields Focused Using Biconcave Acoustic Lens for Normal Incidence
- Urticarial Reaction Caused by Ethanol
- Regulation of Vascular Function by Perivascular Calcitonin Gene-Related Peptide-Containing Nerves
- Preface
- Lafutidine Facilitates Calcitonin Gene-Related Peptide (CGRP) Nerve-Mediated Vasodilation via Vanilloid-1 Receptors in Rat Mesenteric Resistance Arteries
- Effect of Adrenomedullin on Adrenergic Vasoconstriction in Mesenteric Resistance Arteries of the Rat
- LIPO-MICRODIALYSIS : A NEW MICRODIALYSIS METHOD FOR STUDYING THE PHARMACOKINETICS OF LIPOPHILIC SUBSTANCES
- Different Prostanoids Are Involved in Bradykinin-Induced Endothelium-Dependent and-Independent Vasoconstriction in Rat Mesenteric Resistance Arteries
- Long-Term Inhibition of Angiotensin Prevents Reduction of Periarterial Innervation of Calcitonin Gene-Related Peptide (CGRP)-Containing Nerves in Spontaneously Hypertensive Rats
- Decreased Depressor Response Mediated by Calcitonin Gene-Related Peptide(CGRP)-Containing Vasodilator Nerves to Spinal Cord Stimulation and Levels of CGRP mRNA of the Dorsal Root Ganglia in Spontaneously Hypertensive Rats
- Effects of Natriuretic Peptides on the Centrally Mediated Pressor Response to Clonidine in Freely Moving Rats
- Design and Convergence Performance Analysis of Aspherical Acoustic Lens Applied to Ambient Noise Imaging in Actual Ocean Experiment
- Design for Underwater Aplanatic Straubel Acoustic Mirror
- Design for Underwater Aplanatic Acoustic Mirror
- Characterization of the Inhibitory Effect of Vascular Endothelium on Agonist-Induced Vasoconstriction in Rat Mesenteric Resistance Arteries
- Nerve Growth Factor Suppresses Prostate Tumor Growth
- Age-Related Disappearance of the Inhibitory Effect of Vascular Endothelium on Agonist-Induced Vasoconstriction in Rat Mesenteric Vascular Beds
- A Modified Fluorescence Polarization Immunoassay Method Incorporating Fat Emulsion (FE-FPIA) to Determine Cyclosporin A Concentrations in Rat Skin
- UPTAKE AND RELEASE OF 5-HYDROXYTRYPTAMINE IN ADRENERGIC NERVES OF THE RAT MESENTERIC ARTERY
- EFFECTS OF DELTA-9-TETRAHYDROCANNABINOL ON THE CARDIOVASCULAR SYSTEM, AND PRESSOR AND BEHAVIORAL RESPONSES TO BRAIN STIMULATION IN RATS
- CARDIOVASCULAR CHANGES INDUCED BY LARGE DOSES OF CLONIDINE IN MICE
- Effect of Pharmacist Management on Serum Hemoglobin Levels with Renal Anemia in Hemodialysis Outpatients
- DESIGN AND SYNTHESIS OF COLON-SPECIFIC PRO-ANTEDRUGS, 20-GLUCOPYRANOSYLOXY METHYLPREDNISOLONATES, FOR ORAL TREATMENT OF ULCERATIVE COLITIS AND THEIR METABOLISM
- New Molecular Mechanisms for Cardiovascular Disease:Contribution of Endothelium-Derived Hyperpolarizing Factor in the Regulation of Vasoconstriction in Peripheral Resistance Arteries
- New Molecular Mechanisms for Cardiovascular Disease : Contribution of Endothelium-Derived Hyperpolarizing Factor in the Regulation of Vasoconstriction in Peripheral Resistance Arteries
- New Molecular Mechanisms for Cardiovascular Disease : Preface
- Numerical Simulation of Target Range Estimation Using Ambient Noise Imaging with Acoustic Lens
- Evaluating Directional Resolution of Aplanatic Acoustic Lens for Designing Ambient Noise Imaging System
- Fluctuation of Long-Distance Sound Propagation in the Central Pacific
- R-(−)-α-Methylhistamine, a Histamine H3 Receptor Agonist, Induces Endothelium-Dependent Vasodilation in Rat Mesenteric Resistance Arteries
- Estimation of the Travel Time Using the New Measurement Technique for Long-range Transmission
- Small-Scale Trial for Evaluating Directional Resolution of Single Spherical Biconcave Acoustic Lens in Designing of Ambient Noise Imaging System
- Finite Difference Time Domain Analysis of Underwater Acoustic Lens System for Ambient Noise Imaging
- Endothelium-Derived Relaxing Factor-Mediated Vasodilation in Mouse Mesenteric Vascular Beds
- Adrenergic Stimulation-Released Histamine Taken-up in Adrenergic Nerves Induces Endothelium-Dependent Vasodilation in Rat Mesenteric Resistance Arteries
- Anandamide Induces Endothelium-Dependent Vasoconstriction and CGRPergic Nerve-Mediated Vasodilatation in the Rat Mesenteric Vascular Bed
- Involvement of Hypothalamic Periventricular GABAergic Nerves in the Central Pressor Response to Clonidine in Freely-Moving Conscious Rats
- Hyperinsulinemia induces hypertension associated with neurogenic vascular dysfunction resulting from abnormal perivascular innervations in rat mesenteric resistance arteries
- Precise Measurement of Travel Time Difference for Acoustic Reciprocal Transmission
- Extraction of Target Scatterings from Received Transients on Target Detection Trial of Ambient Noise Imaging with Acoustic Lens
- Effect of acrylic cover on convergence property of aplanatic and hyperbolic Fresnel lenses
- Numerical Analysis of Sound Pressure Fields Focused by Phase Continuous Fresnel Lens Using Finite Difference Time Domain Method
- Neurogenic Vascular Responses in Male Mouse Mesenteric Vascular Beds
- Design and Numerical Evaluation of Off-Axis Aplanatic Straubel Mirror for Underwater Acoustic Imaging
- The Mechanism of Calcitonin Gene-Related Peptide-Containing Nerve Innervation
- Neurogenic Vascular Responses in Male Mouse Mesenteric Vascular Beds
- Effects of methamphetamine on the twitch response in the rat isolated vas deferens.
- Analysis of Tidal Effect on Reciprocal Sound Propagation in Central Pacific Ocean
- Analysis of Current Estimation along Sound-Channel Axis in Central Pacific
- Sound Pressure Fields Focused Using Biconcave Acoustic Lens for Normal Incidence
- Consideration of Radiation Impedance Calculation Methods for Cylindrical Array Sound Source
- 3-D FDTD Analysis of Sound Field Focused by Biconcave Acoustic Lens for Normal Incidence
- Design for Aplanatic Fresnel Acoustic Lens for Underwater Imaging
- Relationship between Spatial Distribution of Noise Sources and Target Scatterings Observed in the 2010 Sea Trial of Ambient Noise Imaging
- Design of an Absolutely Aplanatic Acoustic Lens
- Pharmacological characterization of presynaptic .ALPHA.-adrenoceptors in the modulation of the 5-hydroxytryptamine release from vascular adrenergic nerves in the rat.
- Vascular Reactivity to Alpha Adrenoceptor Agonists in Freely Moving SHR and WKY
- Cardiovascular Responses Induced by Intracerebroventricular Injection of Angiotensin III in Freely Moving SHR
- Involvement of the increased calcium influx in positive inotropic action induced by N-ethylmaleimide in the bullfrog ventriculum.
- Intracerebroventricular Treatment with Vasopressin V1-Receptor Antagonist Inhibits Centrally-Mediated Pressor Response to Clonidine in Conscious Rats.
- Central .ALPHA.2-Adrenoceptor-Mediated Pressor Response to Clonidine in Conscious, Spontaneously Hypertensive Rats.
- Effect of neuropeptide Y on vasodilation mediated by Calcitonin Gene-Related Peptide(CGRP)-containing nerves in the mesenteric resistance vessel of the rat.