Purification and Characterization of Sialidase from Porcine Liver

スポンサーリンク

概要

• 論文の詳細を見る

• Sialidase [E.C.3.2.1.18] has previously been purified from porcine liver by procedures including extraction, ammonium sulfate precipitation, concanavalin A-Sepharose adsorption, activation, CM-Sepharose ion exchange chromatography, and HPLC on a Shim pack Diol 300 column. Two sialidase preparations, sialidase I and II, were obtained by CM-Sepharose column chromatography and were eluted with pH 4.5 and 5.0 buffers, respectively. The two enzyme preparations showed the same optimum pH, pH stability, and specificities for natural substrates. The two final preparations contained β-galactosidase activity and showed three protein components of 64,30,and 21 kDa with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which are derived from the β-galactosidase multimer. The anti-β-galactosidase multimer antiserum was able to precipitate sialidase activity. It is likely that porcine liver sialidase exists as a multienzyme complex with β-galactosidase and carboxypeptidase (protective protein).

• 公益社団法人日本薬学会の論文
• 1998-07-15

著者

• 沼沢 光輝

  Tohoku Pharmaceutical University

• FURUHATA KIMIO

  School of Pharmaceutical Sciences, Kitasato University

• Shiraishi Takayuki

  Department of Health Chemistry , Niigata College of Pharmacy

• Nagaoka M

  Tohoku Pharmaceutical Univ. Sendai Jpn

• Nagaoka Masao

  Tohoku College Of Pharmacy:sankyo Co. Ltd.

• SAITOH Mayu

  Department of Anatomy, Toyama Medical and Pharmaceutical University

• UDA Yutaka

  Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy a

• IRIYAMA Naoko

  Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy a

• FURUHATA Kimio

  Scohool of Pharmaceutical Sciences, Kitasato University

• Shiraishi Takayuki

  Laboratoiy Of Health Chemistry Faculty Of Pharmaceutical Science Niigata University Of Pharmacy And

• Uda Y

  Department Of Health Chemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And

• Uda Yutaka

  Department Of Chemical Toxicology Medical Research Institute Tokyo Medical And Dental University

• Saitoh Mayu

  Department Of Anatomy Toyama Medical And Pharmaceutical University

• Saitoh Mayu

  Department Of Health Chemistry Niigata College Of Pharmacy:(present Address)department Of Anatomy Fa

• Iriyama Naoko

  Department Of Health Chemistry Faculty Of Pharmaceutical Sciences Niigata University Of Pharmacy And

• Furuhata K

  Scohool Of Pharmaceutical Sciences Kitasato University

• Furuhata Kimio

  School Of Pharmaceutical Sciences Kitasato University

• NAGAOKA Megumi

  Department of Health Chemistry, Niigata College of Pharmacy

• NAGAOKA Hiroaki

  Department of Health Chemistry, Niigata College of Pharmacy

• 白石 達郎

  Hoechst Japan Co. Ltd.

• Nagaoka Hiroaki

  Department Of Health Chemistry Niigata College Of Pharmacy

• Shiraishi Takayuki

  Department of Electrical and Computer Engineering, Graduate School of Engineering, Yokohama National University, Yokohama 240-8501, Japan

関連論文

• Studies on Sialic Acids. VIII. : The 1,4-Lactone Derivatives of N-Acetylneuraminic Acid(Organic,Chemical)
• Studies on the Granulation Process of Granules for Tableting with a High Speed Mixer. II. Influence of Particle Size of Active Substance on Granulation
• Studies on the Granulation Process of Granules for Tableting with a High Speed Mixer. I. Physical Properties of Granules for Tableting
• アロマターゼの自殺基質ATとシステインとの反応性
• Sea Urchin Sialidase : Partial Purification and Characterization
• C(10)-C(19) Bond Cleavage Reaction of 19-Oxygenated Androst-4-ene-3,6-dione Steroids under Various Conditions
• Improved Synthesis and Molecular Modeling of 4β,19-Dihydroxyandrost-5-en-17-one, an Excellent Inhibitor of Aromatase
• Androst-4-ene-17-thione とその 5-ene 異性体の合成とアロマターゼ阻害活性
• Aromatization of 19-Oxygenated Androst-4-ene-3,6,17-triones with Human Placental Microsomes
• Biochemical Studies of Estr-4-ene-3,6,17-trione and 5α-Androstan-17-ones with or without a Carbonyl Function at C-3 and/or C-6 as Aromatase Inhibitors
• キャピラリーFTD-GCによる玄米および大豆中15種有機含窒素系農薬の一斉分析法
• アロマターゼ自殺基質Androst-4-ene-3,6,17-trioneの19位酸素化体とN-Acetyl-_L-cysteineとの1,4-付加反応
• Synthesis of Deuterium-Labeled 16α, 19-Dihydroxy C_ Steroids as Internal Standards for Gas Chromatography-Mass Spectrometry
• A Radiometric Assay Method for Aromatase Activity Using [1β-^3H]16α-Hydroxyandrostenedione
• Competitive Inhibition and Suicide Inactivation of Human Placental Aromatase by Androst-4-ene-3,6-dione Derivatives and 3α-Methoxyandrost-4-ene-6,17-dione
• Synthesis of 16α-Hydroxyandrost-4-ene-3,17,19-trione and 3β, 16α-Dihydroxyandrost-5-ene-17,19-dione : Potential Intermediates of Estriol Biosynthesis
• Oxygenation of 2,4-Dibromoestrogens with Nitric Acid : A New Synthesis of 19-Nor Steroids
• Radioimmunoassay of 2-Hydroxyestrone Using Antisera Raised against Antigenic Complexs Obtained by Convenient Methods
• Time-Dependent Inactivation of Human Placental Aromatase by Bromoacetoxy 4-Androsten-3-ones in the Presence of Reduced Nicotinamide Adenine Dinucleotide Phosphate (NADPH)
• Reactions of Enolizable Steroidal 4-En-3-ones and 17-Ones with Hypervalent Iodine
• Stereospecific 1,2-Hydride Shift in the Rearrangement of 16β-Hydroxy-17-oxo Steroids to 17β-Hydroxy-16-ones with Acid and Base(Organic,Chemical)
• Preparation of [1,2-^3H,4-^C]16α-Hydroxyandrostenedione and Its Use for Radiometric Determination of Human Placental Aromatase Activity(Analytical,Chemical)
• Regiospecific Deoxygenation of the Dihydroxyacetone Moiety at C-17 of Corticoid Steroids with Iodotrimethylsilane
• Reaction of 21-Bromo-17α-methoxy-20-ketopregnenes with Sodium Methoxide or Potassium Acetate
• シアル酸の化学 : N-アセチルノイラミン酸について
• Development of Diaphragmatic Lymphatics : the Process of Their Direct Connection to the Peritoneal Cavity
• Louisianins A, B, C and D:Non-steroidal Growth Inhibitors of Testosterone-responsive SC 115 Cells II.Physico-chemical Properties and Structural Elucidation
• A Novel Glycosylation of 3-Deoxy-D-glycero-D-galacto-2-nonulosonic Acid (KDN) via in Situ Pyranose-Furanose Rearrangement
• Reaction Products from Fischer's Methylation of 3-Deoxy-D-glycero-D-galacto-2-nonulosonic Acid (KDN)
• Synthesis and Inhibitory Activities of Isochromophilone Analogues against gp120-CD4 Binding
• Photoaffinity Labeling of Sialidase with a Biotin-Conjugated Phenylaminodiazirine Derivative of 2,3-Didehydro-2-deoxy-N-acetylneuraminic Acid(Biochemistry)
• Identification and Characterization of Cathepsin D in a Highly Purified Sialidase from Starfish A. pectinifera
• OCCURRENCE OF LOOSELY BOUND MERCURY IN THE BLOOD OF GUINEA PIG TREATED WITH METHYLMERCURIC CHLORIDE
• GABAergic system in the cochlear nuclei of the rat
• Synthesis of Deuterium-Labelled 16α-Hydroxy-4-androstene-3,17-dione and 16α-Hydroxydehydroepiandrosterone
• Synthesis of Deuterium-labelled Estriol, 16α-Hydroxyestrone and Estriol 16-Glucuronide via 2,4,16α-Tribromoestrone as Internal Standards for Mass Fragmentography
• 急性心筋梗塞のマ-カ-酵素としてのクレアチンキナ-ゼ
• Starfish α-N-Acetylgalactosaminidase:Partial Purification and Characterization
• The Presence of Ceramidase Activity in Liver Nuclear Membrane
• Purification and Characterization of Sialidase from Porcine Liver
• Neighboring Group Participation by Heteroaromatic Rings : The Wagner-Meerwein Type Skeletal Rearrangement in the Electrophilic Addition Reactions of Norbornadiene-Fused Furans, Pyrroles, and Thiophenes
• Effects of Inorganic Anions on the Activation of Acid Sialidases
• Effect of Sulfated Compounds on Acid Sialidase
• Synthesis of Carthamin Acetate, the Red Pigment in Safflower Petals
• Identification of 16α, 19-Dihydroxyandrostenedione in the Serum of Pregnant Women by Gas Chromatography-Mass Spectrometry
• Chemical and Immunological Characterization of the Two α-N-Acetylgalactosaminidases from Squid Liver
• Purification and Characterization of α-N-Acetylgalactosaminidases I and II from the Starfish Asterina amurensis
• DETERMINATION OF AROMATASE ACTIVITY INVOLVED IN THE FORMATION OF ESTRIOL WITH HUMAN PLACENTAL MICROSOMES AND ITS KINETIC PROPERTIES
• Formation of 17β-Alkoxy-16-keto Steroids by Reaction of 16α-Hydroxy-17-keto and 17β-Hydroxy-16-keto Steroids with Trimethylsilyl Iodide in the Presence of Alkyl Alcohols
• Mechanistic Studies of Deoxygenation of Steroidal Ring-D 16,17-Ketols with trimethylsilyl Iodide
• Deoxygenation of Steroidal Ring-D 16,17-Ketols with Trimethylsilyl Iodide
• Synthesis of(+), (-)-Model Compounds and Absolute Configuration of Carthamin ; A Red Pigment in the Flower Petals of Safflower
• High-Speed Atomic Force Microscope by Surface Topography Observer
• The crystal structure of (4R,7S)-7-isopropyl-4-methyl-2-azepanone ((-)-menthone lactam).
• The crystal structure of calcium sulfite tetrahydrate. Interatomic distances and angles.
• The crystal and molecular structure of pikromycin.
• Studies on lactams. VII. Conformation of D-glucono-1,5-lactam.

もっと見る 閉じる

スポンサーリンク