Radioimmunoassay of 2-Hydroxyestrone Using Antisera Raised against Antigenic Complexs Obtained by Convenient Methods

概要

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Antigenic complexes of 2-hydroxyestrone (2-OHE_1) were obtained by Mannich reaction of 2-OHE_1 and bovine serum albumin (BSA) and by coupling of 2-OHE_1 1-glutathione thioether to BSA using glutaraldehyde. Antiserum raised against the antigen obtained by the Mannich reaction had high affinity (K_d=3.8×10^9M^<-1>) and relatively high specificity; cross reactivities for estrone, 4-hydroxyestrone and 2-methoxyestrone were 2.1%, 10% and 1.5%, respectively. The other antiserum also had high affinity (4.5×10^9M^<-1>) but its cross reactivities for the above three steroids were more than 100%. Concentrations of 2-hydroxyestrone in human plasma were determined by radioimmunoassay with the more specific antiserum and Sephadex LH-20 chromatography to be lless than a minimum detectable amount (<10 pg/ml) (men), 20.9 pg/ml (women, proliferation) and 26.0 pg/ml (women, periovulation).
公益社団法人日本薬学会の論文
1989-06-25