Evaluation of Musk by Enzyme-Linked Immunosorbent Assay
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概要
- 論文の詳細を見る
We report the development of enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of a unique musk protein (MP-1) in musk samples. Musk defatted with ethyl acetate/methanol (9:1, v/v) was dipped in cold water and ammonium sulfate was added to the supernatant up to 85% saturation. The resulting precipitate was applied to a Bio-Gel P-100 chromatography. The fraction eluted at the void region was collected and it was consecutively purified by affinity chromatography on a DEAE Affi-Gel Blue and on anion-exchange columns containing DEAE-Sepharose CL-6B. This protein was determined to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions with an apparent molecular weight of 35000 Da and was called as musk protein-1 (MP-1). Polyclonal antibodies of MP-1 were produced by injecting it into a rabbit. These antibodies were reactive to the aqueous extract of musk and the pure antigen. The ELISA could be applied to detect nano gram quantities of the antigen in musk samples. This method made it possible to distinguish musk samples from different origins.
- 公益社団法人日本薬学会の論文
- 2002-04-01
著者
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LEE Jong
Korea Institute of Industrial Technology
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Kim Yeong
Natural Products Research Institute College Of Pharmacy Seoul National University
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Kim Yeong
Natural Products Res. Inst. Coll. Of Pharmacy Seoul National Univ.
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Kim Y
Seoul National Univ. Seoul Kor
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HAHN Bum-Soo
Natural Products Research Institute, Seoul National University
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Cheong Choon
韓国
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Cho So
Natural Products Research Institute College Of Pharmacy Seoul National University:division Of Herbal
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Cho S
Seoul National Univ. Seoul Kor
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Hahn Bum-soo
Natural Products Research Institute Seoul National University
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Kim-pak Youngmi
Department Of Biochemistry Ulsan University College Of Medicine
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JEONG Choon
College of Pharmacy, Duksung Women's University
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AHN Kwang
Natural Products Research Institute, Seoul National University
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CHANG Seung
Korea Food and Drug Administration
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LEE Hyung
Korea Research Institute of Bioscience and Biotechnologyc
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Ahn Kwang
Natural Products Research Institute Seoul National University
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Ahn Kwang
Natural Products Research Institute College Of Pharmacy Seoul National University
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Lee Jong
Korea Food And Drug Administration
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Jeong Choon
College Of Pharmacy Duksung Women 's University
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Lee Jong
Korea Food & Drug Administration
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Lee Hyung
Korea Electronics Technology Institute:school Of Information And Communications Engineering Sungkyun
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