Cytotoxicity of Reactive Oxygen Species and Related Agents Toward Undifferentiated and Differentiated Rat Phenochromocytoma PC12 Cells

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概要

• 論文の詳細を見る

• The cytotoxicity of reactive oxygen species and related agents toward cultured rat adrenal medullary phenochromocytoma PC12 cells was examined. These species and agents include hydrogen peroxide, linoleic acid hydroperoxide (LOOH), tert-butyl hydroperoxide, paraquat, 2, 2'-azobis(2-amidinopropane) dihydrochloride (AAPH), 2-2'-azobis(2, 4-dimethylvaleronitrile)(AMVN), and a hypoxanthine-xanthine oxidase system. The respective 50% lethal concentrations (LC_50) for undifferentiated and differentiated PC12 cells were found to be 275 and 165 μM of hydrogen peroxide, 58.3 and 35.3 μM of LOOH, 536 and 212 μM of tert-butyl hydroperoxide, 42.5 and 26.5 mM of paraquant, 79.5 and 74.5 mM of AAPH, 412 and 300 μM of AMVN, and 37.2 and 16.6 mU・ml^-1 xanthine oxidase activity of the hypoxanthine-xanthine oxidase system. These results show that the differentiated cells were more susceptible to these oxidative agents than the undifferentiated cells. The glutathione peroxidase activity level of the undifferentiated cells was 2-3 times higher than the differentiated cells, the catalase activity level also tended to be higher, the superpxide dismutase activity level was higher on a per-protein-quantity basis but lower on a per-cell-number basis, and the total and reduced glutathione concentration levels were considerably higher. The enhanced susceptibility of the differentiated cells may result from decreases in the activity of glutathione peroxidase and the concentration of its substrate, reduced glutathione (GSH). Further, the preincubation of PC12 cells with α-tocopherol or L-buthionine-(R, S)-sulfoximine (BSO) lowered or enhanced their cytotoxicities, respectively.

• 公益社団法人日本薬学会の論文
• 2001-05-01

著者

• Baba N

  Graduate School Of Natural Science And Technology Okayama University

• Baba Naomichi

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• Baba Naomichi

  Department Of Biore Sources Chemistry Faculty Of Agriculture Okayama University

• Matsuo Mitsuyoshi

  Tokyo Metropolitan Institute of Gerontology

• Sakai Naoko

  Department Of Chemistry Keio University

• Baba Naomichi

  Div. Of Bioscience Graduate School Of Natural Sci. And Technol. Okayama Univ.

• Sasaki N

  Kyoto Univ. Kyoto

• Sasaki Naoko

  Department Of Biology Faculty Of Science And High Technology Research Center Konan University

• MATSUO Mitsuyoshi

  Department of Biology, Faculty of Science and High Technology Research Center, Konan University

• Matsuo Mitsuyoshi

  Department Of Biology Faculty Of Science And High Technology Research Center Konan University

• Matsuo Mitsuyoshi

  Department Of Biology Faculty Of Science Konan University

• BABA Naomichi

  Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University

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