Real-Time Two-Photon Microscopy and Its Application for In Situ Imaging

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概要

• 論文の詳細を見る

• The recent developments in fluorescent-probes and laser technologies have provided us opportunities to observe the biological functions in living organs and tissues under a microscope. Although single-photon excitation confocal microscopy is an indispensable tool for observing these specimens, the observable depth of this microscopy is still not enough to analyze the functions of whole parts of organs in living organisms. To observe the deeper parts, we have attempted to develop new real-time two-photon excitation microscopy equipped with a microlens-arrayed multipinhole scanning device. Using this real-time two-photon microscope, we examined the dynamics of the intracellular calcium concentration([Ca^<2+>]_i)in the cardiac myocytes in the rat whole heart to understand their physiological functions and mechanisms. We could observe Ca^<2+> waves on the surface in the whole heart. However, we still need higher-energy laser power for two-photon excitation and visualization of [Ca^<2+>]_i dynamics in the deeper parts of the whole heart.

• 日本組織細胞化学会の論文

著者

• Tanaka Hideo

  Department of Pathology and Cell Regulation,Kyoto Prefectural University of Medicine

• KAWATA Satoshi

  Department of Chemistry, Graduate School of Science, Osaka University

• NAKAMURA Osamu

  Department of Radiological Technology, Faculty of Health Sciences, Nihon Institute of Medical Scienc

• Oyamada Masahito

  Department of Pathology and Cell Regulation, Kyoto Prefectural Univesity of Medicine

• TAKAMATSU Tetsuro

  Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine

• Oyamada Masahito

  京都府立医科大学 細胞分子機能病理学

• Oyamada M

  Department Of Pathology And Cell Regulation Kyoto Prefectural Univesity Of Medicine

• Oyamada Masahito

  Department Of Pathology And Cell Regulation Kyoto Prefectural University Of Medicine

• Takamatsu T

  Department Of Pathology And Cell Regulation Kyoto Prefectural Univesity Of Medicine

• Kaneko Tomoyuki

  Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine

• Fujita Katsumasa

  Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine

• Kawata S

  Osaka Univ.

• Kawata Satoshi

  Department Of Applied Physics Graduate School Of Engineering Osaka University

• Takamatsu Tetsuro

  Department Of Pathology And Cell Regulation And Department Of Urology Kyoto Prefectural University O

• NAKAMURA Osamu

  Faculty of Environmental Information, Keio University

• Tanaka Hideo

  Department Of Pathology And Cell Regulation Kyoto Prefectural Univesity Of Medicine

• Fujita K

  Department Of Anatomy Saitama Medical School

• Nakamura O

  Faculty Of Environmental Information Keio University

• Tanaka Hideo

  Department Of Hygiene And Preventive Medicine Fukushima Medical University

• Kaneko T

  Department Of Oral And Maxillofacial Surgery Saitama Medical Center Saitama Medical School

• Kawata Satoshi

  Applied Physics Department Faculty Of Engineering Osaka University

• Fujita Keiko

  Department Of Anatomy Saitama Medical University

• Kaneko Tomoyuki

  Department Of Biomedical Information Institute Of Biomaterials And Bioengineering Tokyo Medical And

• Kaneko Tomoatsu

  Pulp Biology And Endodontics Dept. Of Restorative Sciences Graduate School Tokyo Medical And Dental

• Fujita Katsumasa

  Department Of Applied Physics Graduate School Of Engineering Osaka University

• Kawata Satoshi

  Department Of Appkied Physicy Osaka University

• Kaneko Tomoyuki

  Department Of Applied Physics Osaka University

• Tanaka Hideo

  Department Of Hematology And Oncology

• Nakamura Osamu

  Applied Physics Department Faculty Of Engineering Osaka University

• Nakamura Osamu

  Department Of Applied Physics Osaka University

• Tanaka Hideo

  Department Of Applied Chemistry Faculty Of Engineering Okayama University

• KAWATA SATOSHI

  Department of Applied Physics, Graduate School of Engineering, Osaka University

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