Expression of Gap Junctional Protein Connexins in Human Nasal Epithelium, and Its Contribution to Intercellular Calcium Signalling
スポンサーリンク
概要
- 論文の詳細を見る
The purpose of this study was to elucidate what gap junctional protein connexins(Cxs)were expressed in human nasal mucosa and polyps, and to analyze the role of gap junctions in intercellular calcium waves elicited by mechanicial stimulation in cultured human nasal epithelial cells. Immunoconfocal labeling in human nasal tissue showed that Cx26 was expressed between epithelial cells, whereas Cx43 existed in stromal cells. No specific immunolabeling for Cx32 was observed. The capacity of gap junctional intercellular communication in cultured nasal epithelial cells was tested by microinjection with Lucifer yellow, which was transferred into neighboring epithelial cells. To analyze intercellular propagation of calcium waves, cultured nasal epithelial cells were loaded with fluo-3 / AM, and then treated with heptanol(a gap junction inhibitor)or suramin(a P_2-purinergic receptor inhibitor). Both heptanol and suramin reduced intercellular propagation of the calcium wave to 65% and 66%, respectively. Our study indicates that chronic inflammation doesn't significantly influence the pattern of connexin expression in human nasal mucosa, and suggests that calcium waves elicited by mechanical stimulation in cultured human nasal epithelium propagate between epithelial cells through at least two pathways, i.e.gap junctions and P_2-purinergic receptors.
- 日本組織細胞化学会の論文
著者
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TAKENAKA Hiroshi
Department of Earth and Planetary Sciences, Kyushu University
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Oyamada Masahito
Department of Pathology and Cell Regulation, Kyoto Prefectural Univesity of Medicine
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TAKAMATSU Tetsuro
Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine
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Oyamada Masahito
Department Of Pathology And Cell Regulation Kyoto Prefectural University Of Medicine
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Oyamada M
Kyoto Prefectural Univ. Medicine
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Takamatsu Tetsuro
Department Of Pathology And Cell Regulation Kyoto Prefectural University Of Medicine
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Takamatsu Tetsuro
Department Of Pathology And Cell Regulation And Department Of Urology Kyoto Prefectural University O
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Hama Takemitsu
Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine
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Dejima Kenji
Department of Otorhinolaryngology, Kyoto Prefectural University of Medicine
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DEJIMA Kenji
Kyoto Second Red Cross Hospital
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Takenaka Hiroshi
Department Of Otolaryngology Osaka Medical College
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Takenaka Hiroshi
Department Of Otorhinolaryngology Osaka Medical College
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Hama Takemitsu
Department Of Otorhinolaryngology-head And Neck Surgery Kyoto Prefectural University Of Medicine
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Takenaka H
Kyushu Univ.
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Takenaka Hiroshi
Department Of Earth And Planetary Sciences Kyushu University
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Dejima Kenji
Department Of Otolaryngology Kyoto Second Red Cross Hospital
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Takenaka Hiroshi
Department Of Chemistry Faculty Of Science Osaka City University
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