Temporal Alteration of Connexin43 Localization during Ultraviolet Light-induced Apoptosis

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Although gap junctions are thought to play an important role in moderating apoptosis, the basic phenomena underlying when and where the alterations of gap junctions occur during apoptosis have not been well documented. In this study we analyzed the spatiotemporal changes of connexin (Cx) during UV light-induced apoptosis using Cx43-enhanced green fluorescent protein (EGFP)-expressing He La cells, and compared them with those of mitochondrial membrane potential (MMP) using tetra-methylrhodamine ethyl ester (TMRE) and nuclear morphological observation using Hoechst 33342. At 2 hr post-UV-irradiation, a third of the cells were TMRE negative, i.e., they showed the loss of MMP, but with slight nuclear fragmentation, and high percentages of linear Cx43-EGFP plaques were found among both TMRE-positive and TMRE-negative cells. At 4 hr post-UV-irradiation, the percentage of these linear plaques was decreased, and both punctate and diffuse localization of Cx43-EGFP were noted in the cytoplasm of TMRE-negative cells without nuclear fragmentation. At 8 hr post-irradiation, punctate cytoplasmic localization of Cx43-EGFP was noted in TMRE-negative cells with nuclear fragmentation. Treatment with the caspase inhibitor Z-VAD-FMK blocked nuclear fragmentation and partially preserved both gap junctional plaques and MMP. These results indicate that, during apoptosis, Cx mobilization into the cytoplasm occurs after MMP depolarization but before nuclear fragmentation and that this alteration partly depends on caspase.
日本組織細胞化学会の論文

Temporal Alteration of Connexin43 Localization during Ultraviolet Light-induced Apoptosis

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