Isolation and Purification of Cytochrome b_<561> from a Photosynthetic Bacterium, Rhodopseudomonas sphaeroides
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概要
- 論文の詳細を見る
Cytochrome b_<561> was removed from chromatophores of a photoanaerobically grown Rhodopseudomonas sphaeroides by deoxycholate-cholate and Triton X-100 treatments of the chromatophores. The cytochrome was purified by ammonium sulfate fractionation and gel filtration. Its molecular weight was 45,000 (45 kD) and it was composed of three subunits with molecular weights of 23 kD, 19 kD and less than 6 kD. The cytochrome preparation had absorption maxima at 414nm in the oxidized form, and at 428, 530 and 561 nm in the reduced form. Its pI was 4.8. The midpoint potential of this cytochrome was 153 mV at pH 7.0. The compound was autooxidizable, and it had cytochrome c oxidase activity.
- 日本植物生理学会の論文
著者
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Tanaka Hideo
Department of Pathology and Cell Regulation,Kyoto Prefectural University of Medicine
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Takamiya Ken-ichiro
Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Techn
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Takamiya Ken-ichiro
Department Of Biology Faculty Of Science Kyushu University 33
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Takamiya Ken-ichiro
Department Of Biological Sciences Faculty Of Bioscience And Biotechnology
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Tanaka Hideo
Department Of Biology Faculty Of Science Kyushu University 33
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Tanaka Hideo
Department Of Applied Chemistry Faculty Of Engineering Okayama University
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