THE PRINCIPLE AND APPLICATIONS OF OPTICAL MICROSCOPE TOMOGRAPHY (<Symposium>Multiparametric Photometry and Histo-and Cytochemistry)

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概要

• 論文の詳細を見る

• A new principle to obtain sectional images of a thick specimen by a conventional optical microscope without physically slicing is developed. An optical microscope with an off-axis pupil is used to project a three-dimensional (3-D) distribution of a specimen in various directions within the numerical aperture of the objective lens onto a fixed TV camera position. The obtained images of the thick sample are combined to reconstruct its 3-D density distribution. Since the system is strictly angularly-limited, a strong constraint is needed to form the 3-D structure with a moderate spatial resolution from the projections. The conjugate gradient method with the object boundary constraint which is a priori known solves this problem. Experimental results with a biological sample verify the capability of the proposed method as a promising instrumentation of optical tomography in microscopic size.

• 日本組織細胞化学会の論文

著者

• KAWATA Satoshi

  Department of Chemistry, Graduate School of Science, Osaka University

• Kawata S

  Osaka Univ.

• Kawata Satoshi

  Department Of Applied Physics Graduate School Of Engineering Osaka University

• MINAMI Shigeo

  Department of Applied Physics, Osaka University

• Minami Shigeo

  Department Of Applied Physics Faculty Of Engineering Osaka University

• Minami Shigeo

  Department Of Applied Physics Osaka University

• Kawata Satoshi

  Applied Physics Department Faculty Of Engineering Osaka University

• Kawata Satoshi

  Department Of Appkied Physicy Osaka University

• KAWATA SATOSHI

  Department of Applied Physics, Graduate School of Engineering, Osaka University

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