IMMUNOCYTOCHEMICAL LOCALIZATION OF S-100 PROTEIN AND CALMODULIN IN C6 GLIOMA CELLS
スポンサーリンク
概要
- 論文の詳細を見る
The localization of S-100, calmodulin and tubulin in the cultured C6 glioma cells was observed with the indirect immunoperoxidase method. The direct immunoperoxidase method for S-100 was also employed. The reaction products for S-100, calmodulin and tubulin were all found over filamentous structures in the interphase cells. Calmodulin and tubulin seemed to be localized in the cytoplasm, while S-100 was exhibited as intense granular reaction product in the nuclei. In the mitotic cells, these proteins were shown to be present with characteristic localization in the mitotic apparatus. Calmodulin-specific staining was highly intense at the spindle poles. In contrast, S-100 was shown to be concentrated on the chromosomes in all phases of mitotic cells. The direct method showed more accurate and defined localization of S-100 than the indirect method. This is the first demon-stration of the possible interrelationship between S-100 protein and chromosome, although the biological significance of the fact is not known at present.
- 日本組織細胞化学会の論文
著者
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KATO Kanefusa
Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony
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Sugitani Masahiko
日本大学 医学部心臓血管外科学分野
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Sano M
Department Of Pathology School Of Medicine Nihon University
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Sano Makoto
日本大学 医学部病理学
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Kato Keitaro
Department Of Physiological Chemistry Faculty Of Pharmaceutical Sciences Kyushu University
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Seto‐ohshima A
Aichi Prefectural Colony Kasugai
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Seto‐ohshima A
Institute For Developmental Research Aichi Human Service Center:laboratory For Behavioral Genetics B
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Seto-ohshima Akiko
Department Of Morphology Institute For Developmental Research Aichi Prefectural Colony
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Seto-ohshima Akiko
Department Of Morphology Institute For Developmental Research Aichi Human Service Center
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Kato Kanefusa
Department Of Biochemistry Institute For Developmental Research
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Sano Makoto
Department Of Pathology Keio University School Of Medicine
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SANO Mamoru
Department of Biology, Faculty of Medicine, Kyoto Prefectural University of Medicine
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Sano Mamoru
Department Of Biology Faculty Of Medicine Kyoto Prefectural University Of Medicine
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Mizutani Akira
Institute For Developmental Research Aichi Prefectural Colony
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Mizutani Akira
Department Of Morphology Institute For Developmental Research Aichi Prefectural Colony
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Mizutani Akira
Aburahi Laboratories Shionogi & Co. Ltd.
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Mizutani Akira
Department Of Cytochemistry Chest Disease Research Institute Kyoto University
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