Cleavage of calnexin caused by apoptotic stimuli: Implication for the regulation of apoptosis
スポンサーリンク
概要
- 論文の詳細を見る
Calnexin is an endoplasmic reticulum (ER)-resident molecular chaperone that plays an essential role in the correct folding of membrane proteins. We found that calnexin is subjected to partial cleavage in apoptotic mouse cells. Both ER stress-inducing and ER stress-non-inducing apoptotic stimuli caused the cleavage of calnexin, indicating that this event does not always occur downstream of ER stress. The inhibition of caspases that target the amino acid sequence DXXD abrogated calnexin cleavage in apoptotic stimulus-treated cells. In addition, disruption of one of two DXXD sequences located in the cytoplasmic domain caused calnexin to escape cleavage during apoptosis. Furthermore, calnexin was cleaved in vitro by recombinant caspase-3 or caspase-7. Finally, the overexpression of a presumed cleavage product of calnexin partly inhibited apoptosis. These results collectively suggest that caspase-3 or caspase-7 cleaves calnexin, whose cleaved product leads to the attenuation of apoptosis.
- 日本生化学会 = Japanese Biochemical Societyの論文
- 2004-09-01
著者
-
KATO Kanefusa
Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony
-
NAKANISHI Yoshinobu
Graduate School of Natural Science and Technology, Kanazawa University
-
Kato Kanefusa
Department Of Biochemistry Institute For Developmental Research
-
Nakanishi Yoshinobu
Graduate School Of Medical Science Kanazawa University
-
Kato Kanefusa
Department Of Molecular Neurobiology Institute For Developmental Research Aichi Human Service Center
-
TAKIZAWA Takenori
Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Cent
-
TATEMATSU Chizuru
Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Cent
-
WATANABE Kimi
Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Cent
-
Watanabe Kimi
Department Of Molecular Neurobiology Institute For Developmental Research Aichi Human Service Center
-
Nakanishi Y
Faculty Of Pharmaceutical Sciences Kanazawa University
-
Takizawa T
Aichi Human Serv. Center Aichi
-
Takizawa Takenori
Department Of Biochemistry Institute For Developmental Research Aich Human Service Center
-
Tatematsu C
Aichi Human Serv. Center Aichi
-
Tatematsu Chizuru
Department Of Biochemistry Institute For Developmental Research Aichi Human Service Center
-
Tatematsu Chizuru
Department of Biochemistry and Institute for Deveclopmental Research. Aichi Human
関連論文
- SERUM β-ENOLASE IN THE CLINICAL DIAG NOSIS OF ACUTE MYOCARDIAL INFARCTION : Ischemic Heart Disease (V) : FREE COMMUNICATIONS (V) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Comparison of Calbindin D-28k and S-100 Protein B in Neuroblastoma as Determined by Enzyme Immunoassay
- MANGANESE-CONTAINING SUPEROXIDE DISMUTASE IN BLOOD AND URINE DURING OPEN-HEART SURGERY
- CREATINE KINASE BB ISOZYME IN THE BLOOD DURING OPEN HEART SURGERY : Comparison with Creatine Kinase MB and MM Isozymes
- -410- CHANGES OF THE SERUM CONCENTRATIONS OF S100a_0 PROTEIN IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION
- SERIAL DATERMINATIONS OF SERUM β-ENOLASE DURING CARDIAC SURGERY : Circulatory Assistance : PROCEEDINGS OF THE 50th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Inhibitory effect of N-palmitoylphosphatidylethanolamine on macrophage phagocytosis through inhibition of Rac1 and Cdc42
- Modulation of the Stress-Induced Synthesis of Stress Proteins by a Phorbol Ester and Okadaic Acid^1
- Enhancement of Stress- Induced Synthesis of Stress Proteins by Mastoparan in C6 Rat Glioma Cells^1
- Inhibition of Proteasomes Induces Accumulation, Phosphorylation, and Recruitment of HSP27 and αB-Crystallin to Aggresomes
- The testicular fatty acid binding protein PERF15 regulates the fate of germ cells in PERF15 transgenic mice
- Ultrastructural Observation of Gamma-enolase-positive Cells in Human Epidermis
- Cleavage of calnexin caused by apoptotic stimuli: Implication for the regulation of apoptosis
- Brain-derived neurotrophic factor, nerve growth factor and neurotrophin-3 in selected regions of the rat brain following kainic acid-induced seizure activity
- G Protein γ Subunits Coimmunoprecipitated with Antibodies against α Subiunits: Identification of Major Isoforms in Cultureed Cells by Silver Stain and Immunoblotting with Conventional Transfer Procedure
- Induction of the Synthesis of hsp27 and αB Crystallin in Tissues of Heat-Stressed Rats and Its Suppression by Ethanol or an α_1-Adrenergic Antagonist
- The G-Protein G_0 in Mammalian Cardiac Muscle: Localization and Coupling to A_1 Adenosine Receptors
- Ectopic activation of the transcription promoter for the testis-specific mouse Pgk-2 gene on elimination of a cis-acting upstream DNA region
- CHANGES OF CALMODULIN AND S-100 PROTEIN IN C6 GLIOMA CELLS AS A RESULT OF CELLULAR DIFFERENTIATION INDUCED BY FORSKOLIN
- IMMUNOCYTOCHEMICAL LOCALIZATION OF S-100 PROTEIN AND CALMODULIN IN C6 GLIOMA CELLS
- Distribution of γ-Aminobutyric Acid (GABA) and the Calcium-binding Protein Parvalbumin in Rat Retina During Development
- Immunohistochemical and Immunochemical Localizaiton of Heat Shock Protein 27KD in the Developing Rat Retina: The Differential
- Neurotrophic and neuroprotective effects of neuron-specific enolase on cultured neurons from embryonic rat brain
- Structural change of ribosomes during apoptosis: Degradation and externalization of ribosomal proteins in doxorubicin-treated Jurkat cells
- Age-related changes in levels of brain-derived neurotrophic factor in selected brain regions of rats, normal mice and senescence-accerated mice: a compatison to those of nerve growth factor and neurotrophin-3
- EFFECTS OF REPETITIVE RECYCLING OF IMPULSE TRANSMISSION IN THE GASTROCNEMIUS OF NEOSTIGMINE TREATED RATS
- Phosphatidylserine- and integrin-mediated phagocytosis of apoptotic luteal cells by macrophages of the rat
- Expression and function of class B scavenger receptor type I on both apical and basolateral sides of the plasma membrane of polarized testicular Sertoli cells of the rat
- Phagocytic Removal of Apoptotic Spermatogenic Cells by Sertoli Cells : Mechanisms and Consequences
- A presumed human nuclear autoantigen that translocates to plasma membrane blebs during apoptosis
- Independence of plasma membrane blebbing from other biochemical and biological characteristics of apoptotic cells
- Stimulation of Phagocytosis of Influenza Virus-Infected Cells through Surface Desialylation of Macrophages by Viral Neuraminidase
- Signalling pathway involving GULP, MAPK and Rac1 for SR-BI-induced phagocytosis of apoptotic cells.
- Recruitment of Apoptotic Cysteine Proteases(Caspases)in Influenza Virus-Induced Cell Death
- Isolation of a Drosophila gene coding for a protein containing a novel phosphatidylserine-binding motif
- Double-stranded RNA-activated protein kinase (PKR) fused to green fluorescent protein induces apoptosis of human embryonic kidney cells: Possible role in the Fas signaling pathway
- Three Leucine-Rich Sequences and the N-Terminal Region of Double-Stranded RNA-Activated Protein Kinase (PKR) Are Responsible for Its Cytoplasmic Localization^1
- Concomitant induction of apoptosis and expression of monocyte chemoattractant protein-1 in cultured rat luteal cells by nuclear factor-κB and oxidative stress
- Influenza virus and apoptosis : the role of double-stranded RNA-activated protein kinase
- Masking of a dystrophic symptom in genotypically dystrophic-dwarf mice.
- G Protein .GAMMA. Subunits Coimmunoprecipitated with Antibodies against .ALPHA. Subunits: Identification of Major Isoforms in Cultured Cells by Silver Stain and Immunoblotting with Conventional Transfer Procedure.
- Double-Stranded RNA-Activated Protein Kinase(PKR) Fused to Green Fluorescent Protein Induces Apoptosis of Human Embryonic Kidney Cells: Possible Role in the Fas Signaling Pathway.
- Inhibition of Proteasomes Induces Accumulation, Phosphorylation, and Recruitment of HSP27 and .ALPHA.B-Crystallin to Aggresomes.