LEAD AFFINITY OF NUCLEAR PORES-POSSIBLE EXISTENCE OF LIPID-LIKE SUBSTANCE
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When the frozen sections of glutaraldehyde- or formol-fixed tissues were incubated in lead solution and, then, converted to sulfide, marked lead staining was occurred at most of the nuclear pores in fine structural level. This characteristic seemed to be general in various cells from mammals to cold blooded animals. The organs examined were the rat liver, heart, kidney, lung and pancreas, transplanted mammary tumor of mouse, and the liver of trout. Testing with the Markham's rotation technique, the lead precipitate was mostly localized in inner and inter space of the lamellar subunits, and moderately in outside perimeter. This affinity of nuclear pore to lead ions was abolished with pretreatment of the sections in organic solvents and Triton-X-100 suggesting lipid-like nature which might regulate the interaction between nucleoplasm and cytoplasm.
- 日本組織細胞化学会の論文
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- LEAD AFFINITY OF NUCLEAR PORES-POSSIBLE EXISTENCE OF LIPID-LIKE SUBSTANCE