CYTOCHEMICAL DEMONSTRATION OF GUANYLATE CYCLASE ACTIVITY IN RETINAL PHOTORECEPTORS WITH SPECIAL REFERENCE TO CHANGES UNDER LIGHT AND DARK ADAPTATION

概要

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The localization of guanylate cyclase activity was studied cytochemically in the rod outer segments of rats light and dark-adapted for various intervals. A brown colored reaction was detected in rod outer segments under a light microscope. Under an electron microscope, a significant change in the distribution of the reaction products under light and dark adaptation was observed. When rats were dark-adapted for longer than 90 min, an intense activity was found on the cytoplasmic side of the plasma membrane, and most of the disc membranes showed lower activity than in light with the exception of a few disc membranes near the pigment epithelium which showed rather intense activity. At 1 sec illumination, no significant change was found in the distribution of the guanylate cyclase. The first significant change was detected at 5 sec of illumination. After a 15 sec light exposure, the reaction products of guanylate cyclase activity were observed on all the disc membranes regularly, and the activity on the plasma membrane was very weak. The present results indicate that light affects the guanylate cyclase activity and its distributionin the rod outer segments, suggesting that guanylate cyclase or cyclic GMP plays an important role in the control of ion permeability in the rod outer segment plasma membrane and a possible role in the visual adaptation process.
日本組織細胞化学会の論文