ULTRACYTOCHEMICAL DEMONSTRATION OF NADPH-FERRICYANIDE REDUCTASE ACTIVITY
スポンサーリンク
概要
- 論文の詳細を見る
As an extension of our studies on the copper ferrocyanide method for the ultracytochemical demonstration of oxidoreductase activity, the method for the detection of NADPH-ferricyanide reductase activity was developed. The incubation medium consists of 20 mg of NADPH, 13.0 ml of 0.1 M phosphate buffer, pH 7.0, 1.3 ml of 0.1 M sodium citrate, 2.0 ml of 30 mM copper sulfate, 1.7 ml of double distilled water, 2.0 ml of 5 mM potassium ferricyanide and 3.0 g of sucrose. It was observed in the preliminary experiment that potassium sodium tartrate caused more diffusion of the final reaction products than sodium citrate. Fresh tissues from brain, liver, heart and kidney of normal adult rats were used. Formaldelyde inhibited the enzymatic activity completely. Incubation with medium was carried out for 30 minutes at 37℃ under aerobic and anaerobic conditions. After the incubation specimens were fixed in 1% osmium tetroxide and processed for electron microscopy. The NADPH-ferricyanide reductase activity was extremely low in all tissues examined. In the enzymatically positive tissues the activity was observed solely in mitochondria. No activity was demonstrated in any element of the endoplasmic reticulum. The enzymatic activity in specimens incubated under anaerobic conditions was slightly higher in terms of the number of enzymatically positive mitochondria and the rate of positive reaclion in each mitochondrion than that incubated under aerobic conditions. In mitochondria the reaction product was observed in the membrane system including the outer membrane, the intracristal space and the outer space. In some areas, the activity in the outer membrane was quite evident. No activity was observed in the mitochondrial matrix.
- 日本組織細胞化学会の論文
- 1971-11-00
著者
-
Ago Yasuhiro
Department Of Anatomy Kansai Medical School
-
Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
関連論文
- PJ-750 Current Smoking is the Most Important Risk Factor with First Diagnosed Acute Coronary Syndrome(PJ126,Smoking/Alcohol/Life Style (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- The Chloroplast Nucleus : Distribution, Number, Size, and Shape, and a Model for the Multiplication of the Chloroplast Genome during Chloroplast Development
- Usefulness of a Second Temporary Vena Cava Filter for Preventing Acute Pulmonary Thromboembolism
- USE OF AMP (2-AMINO-2-METHYL-1-PROPANOL) BUFFER FOR HISTOCHEMICAL DEMONSTRATION OF ALKALINE PHOSPHATASE ACTIVITY
- USE OF GLYCINE BUFFER FOR THE LEAD CITRATE METHOD OF NUN-SPECIFIC ALKALINE PHOSPHATASE CYTOCHEMISTRY
- Modified Lead Citrate Method for the Demonstration of Non-specific Alkaline Phosphatase
- 養殖マダイとイシガキダイの粘液胞子虫性やせ病
- ULTRACYTOCHEMICAL LOCALIZATION OF CA^-ATPase ACTIVITY ON THE EPITHELIAL CELLS OF RAT TRACHEA
- A NEW ONE-STEP METHOD FOR THE HISTOCHEMISTRY AND CYTOCHEMISTRY OF CA^-ATPASE ACTIVITY
- Histochemical and Cytochemical Demonstration of Ca^-ATPase Activity in Various Tissues of Rat
- IMMUNOHISTOCHEMICAL ASSESSMENT OF SUPEROXIDE DISMUTASE EXPRESSION IN THE HUMAN ENDOMETRIUM THROUGHOUT THE MENSTRUAL CYCLE
- THE "MICROSLICER", A NEW INSTRUMENT FOR MAKING NON-FROZEN SECTIONS
- USE OF TRICINE BUFFER FOR ENZYME HISTOCHEMICAL DEMONSTRATION OF ALKALINE PHOSPHATASE ACTIVITY BY LEAD CITRATE METHOD
- CYTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASE IN NORMAL AND CATARACTOUS RAT LENSES
- MORPHOMETRIC ANALYSIS OF GAP JUNCTIONS IN THE RAT LENS DURING CATARACT FORMATION
- ULTRASTRUCTURE AND Ca^-ATPASE ACTIVITY OF GAP JUNCTIONS UNDER CRYO-ELECTRON MICROSCOPIC OBSERVATION
- AN ATTEMPT TO CLARIFY THE CONTROL MECHANISM OF AUTOPHAGY
- CATHODOLUMINESCENCE ELECTRON MICROSCOPY FOR CYTOCHEMICAL STUDIES AT THE ORGANELLE LEVEL
- Effects of the Noncyclic Cyanamides on the Retrogradation of Waxy Corn Starch
- Simple Determination Method of Degree of Substitution for Starch Acetate
- APPLICATION OF ANALYTICAL COLOR FLUORESCENCE ELECTRON MICROSCOPY TO BIOMEDICAL FIELD: II. CHOLESTEROL ESTER IN RAT ADRENAL CORTEX
- APPLICATION OF ANALYTICAL COLOR FLUORESCENCE ELECTRON MICROSCOPY TO BIOMEDICAL FIELD: I. VITAMIN A ESTER IN RAT RETINA
- FODRIN IS LOCALIZED IN THE CYTOPLASM OF KERATINOCYTES CULTURED IN LOW CALCIUM MEDIUM : IMMUNOELECTRON MICROSCOPIC STUDY
- DISTRIBUTION OF FODRIN AND α-ACTININ IN THE RAT RETINAL PIGMENT EPITHELIUM IN VIVO
- CYTOCHEMICAL DEMONSTRATION OF LECTIN BINDING SITES IN ROD PHOTORECEPTOR CELLS AND INTERPHOTORECEPTOR MATRIX OF RAT RETINAS BY LABELING FROZEN THIN-SECTIONS WITH VARIOUS LECTINS
- THREE-DIMENSIONAL DISTRIBUTION OF ACID PHOSPHATASE-POSITIVE THREAD-LIKE STRUCTURES "NEMATOLYSOSOMES" IN RAT PANCREATIC EXOCRINE CELLS
- DOUBLE STAINING PROCEDURE FOR HISTOCHEMICAL LOCALIZATION OF ALCOHOL AND ALDEHYDE DEHYDROGENASE ACTIVITIES IN THE MOUSE LIVER
- HISTOCHEMICAL LOCALIZATION OF ALDEHYDE DEHYDROGENASE ACTIVITY IN THE MOUSE LIVER BY THE COPPER FERROCYANIDE METHOD
- Distribution of Fodrin in Chromaffin Cells of the Rat Adrenal Medulla during Secretion
- CYTOPLASMIC OCCURRENCE OF FODRIN IN THE PRINCIPAL CELL OF THE RENAL COLLECTING TUBULE, THE EPIDERMAL KERATINOCYTE AND THE ADRENAL CHROMAFFIN CELL (Histo- and Cytochemistry in Tissue Culture)
- DISTRIBUTION OF FODRIN IN PAM 212 CELLS CULTURED IN LOW AND STANDARD Ca^
- IMMUNOFLUORESCENCE MICROSCOPIC STUDY OF FODRIN, TUBULIN AND F-ACTIN IN THE RAT KERATINOCYTES
- Na-K-ATPase Localization in the Rat Paw Eccrine Sweat Gland
- ULTRASTRUCTURAL LOCALIZATION OF OUABAIN-SENSITIVE POTASSIUM-DEPENDENT P-NITROPHENYLPHOSPHATASE (NA^+-K^+-ATPASE) ACTIVITY IN THE CENTRAL NERVOUS SYSTEM OF THE RAT
- The Movement of Cell Membrane Materials in Isolated Frog Urothelial Cells Labeled by Horseradish Peroxidase (HRP)
- CA^-ATPASE ACTIVITY IN THE GASTRIC PARIETAL CELLS OF RAT DURING PERINATAL DEVELOPMENT
- UNEVEN FORMATION OF FILIPIN-STEROL COMPLEXES IN FROG URINARY BLADDER EPITHELIUM
- COLOR CATHODOLUMINESCENCE IMAGES AND CATHODOLUMINESCENCE SPECTRA ANALYSIS OF BIOLOGICAL MATERIALS
- NEW CYTOCHEMICAL METHOD FOR ADENYLATE CYCLASE AND GUANYLATE CYCLASE WITH DIMETHYL SULFOXIDE
- Use of parasites as biological tags for separating stocks of the starpotted dogfish Mustelus manazo in Japan and Taiwan
- REGIONAL DIFFERENCES OF AN NAD(P)H-DEPENDENT H_2O_2-GENERATING ENZYME ACTIVITY IN THE RAT SMALL INTESTINE
- POSTNATAL CHANGES IN THE LOCALIZATION OF SMALL INTESTINAL NAD(P)H-DEPENDENT H_2O_2-GENERATING ENZYME ACTIVITY IN THE RAT
- CYTOCHEMICAL LOCALIZATION OF GLUCOSE 6-PHOSPHATASE ACTIVITY IN THE LIVER OF GROWING MICE AFTER PHENOBARBITAL TREATMENT
- Survey of Ca^-Dependent Nuclease in Green Plants
- Myxosporean and microsporidian infections in cultured Pacific bluefin tuna Thunnus orientalis in Japan
- Chinese Traditional Medicine Protects Gastric Parietal Cells from Adriamycin Cytotoxicity
- Immunocytochimical Evidence for Interleukin-2 Receptor Regulation on Rat Anterior Pituitary and Adrenal Cells
- Morphological differences between larvae and in vitro-cultured adults of Anisakis simplex (sensu stricto) and Anisakis pegreffii (Nematoda : Anisakidae)
- Myxobolus buri, the myxosporean parasite causing scoliosis of yellowtail, is synonymous with Myxobolus acanthogobii infecting the brain of the yellowfin goby
- CHANGES IN INTRACISTERNAL PEROXIDASE ACTIVITY DURING ALVEOLAR EPITHELIAL DIFFERENTIATION IN MOUSE LUNGS
- ELECTRON CYTOCHEMICAL STUDIES ON AN NAD(P)H-DEPENDENT H_2O_2-GENERATING ENZYME ACTIVITY IN THE RAT SMALL INTESTINE
- Two Primary Melanomas of the Human Brain
- EFFECT OF HEMODYNAMIC FACTORS IN RENAL GLOMERULUS II. CYTOCHEMISTRY OF GLOMERULAR BASEMENT MEMBRANE AND MESANGIUM IN THE EXPERIMENTAL CONGESTIVE KIDNEY
- The Intracellular Calcium Store Function of Platelet Granules
- THE ROLE OF SCHWANN CELLS AND MACROPHAGES IN THE REMOVAL OF MYELIN DURING WALLERIAN DEGENERATION
- ULTRASTRUCTURAL AND CYTOCHEMICAL OBSERVATIONS ON HETEROPHAGY AND AUTOPHAGY OF MACROPHAGE IN MOUSE THYMUS
- Atrial Specific Granules : An Intracellular Calcium Store
- THE RELATIONSHIP BETWEEN HETEROPHAGY AND AUTOPHAGY IN THE SPLENIC MACROPHAGE OF RATS AFTER γ-RAY IRRADIATION
- Studies on the Retrogradation and Structural Properties of Waxy Corn Starch
- Cytochemical Demonstration of Adenylate Cyclase and Guanylate Cyclase Activities with Dimethyl Suloxide
- ELECTRON-CYTOCHEMICAL STUDIES ON THE DIFFERENTIATION OF MOUSE LUNG ALVEOLAR EPITHELIAL CELLS WITH SPECIAL REFERENCE TO CHANGES IN MITOCHONDRIA
- Occurrence of Caligus lalandei Barnard, 1948 (Copepoda, Siphonostomatoida) on Amberjacks (Seriola spp.) in the Western North Pacific(Animal Diversity and Evolution)
- AUTOPHAGY OF PEROXISOMES IN HEPATIC PARENCHYMAL CELLS
- HISTOCHEMICAL AND CYTOCHEMICAL STUDIES OF NEUTRAL pH p-NPPase ON LYSOSOMAL MEMBRANE IN RAT LIVER
- Protection Against The Side Effect of Mitomycin C by Coenzyme Q_ in Mouse Liver
- PROTECTIVE EFFECT OF COENZYME Q_ AGAINST THE ADVERSE REACTION OF MITOMYCIN C IN MOUSE LIVER
- DIRECT VISUALIZATION OF THE MEMBRANE-ASSOCIATED COATED VESICLES AND CYTOSKELETON IN CULTURED MACROPHAGES BY A NEW MEANS OF DRY-BLOWING
- Direct Visualization of Coated Vesicles in Critical Point-dried Macrophages
- IN SITU AND IN VITRO MORPHOLOGICAL EVIDENCE FOR THE INTERACTION OF ACTIN FILAMENTS WITH LYSOSOMES IN RAT ALVEOLAR MACROPHAGES
- REGULATION OF INTRACELLULAR LYSOSOMAL MOVEMENTS BY THE CYTOSKELETAL SYSTEM IN RAT ALVEOLAR MACROPHAGES
- ULTRACYTOCHEMISTRY OF CHOLINE ACETYLTRANSFERASE IN THE NEOSTRIATUM OF THE RAT BRAIN
- ENZYME CYTOCHEMICAL AND MORPHOMETRICAL ANALYSES OF GAP JUNCTIONS IN RABBIT OVARIAN GRANULOSA CELLS DURING FOLLICLE MATURATION AND OVULATORY PROCESS
- CYTOCHEMICAL LOCALIZATION OF ACID PHOSPHATASE IN THE RABBIT OVARIAN FOLLICLE
- EFFECT OF ETHANOL ADMINISTRATION ON ALCOHOL DEHYDROGENASE ACTIVITY IN MOUSE HEPATIC CELLS : ULTRACYTOCHEMICAL STUDY
- Effect of Ethanol Administration on Alcohol Dehydrogenase Activity in Mouse Hepatic Cells
- H_2O_2 Generation Stimulated by Phorbol Myristate Acetate in Mouse Neutrophils and Cultured Activated Macrophages
- Alterations of Glomerular Basement Membrane in the Experimental Congestive Kidney
- Intracellular Immunoglobulin (Ig) in Chronic Lymphocytic Leukemic (CLL) Cells and PWM-induced Responses
- CYTOCHEMICAL STUDIES ON THE EFFECT OF INTRAPERITONEAL AND ORAL ADMINISTRATION OF A TRADITIONAL CHINESE MEDICINE (SHO-SAIKO-TO) ON THE D-GALACTOSAMINE-INDUCED HEPATIC INJURY OF RATS
- ULTRACYTOCHEMICAL LOCALIZATION OF CA^-ATPASE ACTIVITY IN THE EPITHELIAL CELLS OF GUINEA PIG CILIARY BODY
- ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT p-NITROPHENYLPHOSPHATASE ACTIVITY IN GUINEA PIG RETINA : II. NEURONS AND MULLER CELLS
- Cytochemical Studies on NAD(P)H Oxidase Activity in Cultured Retinal Pigment Epithelial Cells
- A HISTOCHEMICAL STUDY ON THE BLOOD-OPTIC NERVE AND FLUID-OPTIC NERVE BARRIER
- Studies on the Blood-optic and the Liquor-optic Barriers using Horseradish Peroxidase as a Tracer
- Speciation of the Paradeontacylix spp. (Sanguinicolidae) of Seriola dumerili. Two new species of the genus Paradeontacylix from the Mediterranean
- Evaluation of Time-Dependent Fracture Mechanics Parameters of a Moving Crack in a Viscoelastic Strip
- REGULATORY FACTORS OF INTRACELLULAR LYSOSOMAL ACTIVITY ASSOCIATED WITH HETEROPHAGY AND AUTOPHAGY (Functional Morphology and Pathology of Lysosomes)
- CALCIUM-ACTIVATED ADENOSINE TRIPHOSPHATASE AND GAP JUNCTIONS IN RAT EPIDERMIS
- PJ-244 The out comes of third day may determine clinical course for AMI(Acute Myocardial Infarction, Clinical (Diagnosis/Treatment) 9 (IHD) : PJ41)(Poster Session (Japanese))
- Different Actions of Chinese Traditional Medicines against Adriamycin-induced Cytotoxicity
- GOLGI APPARATUS IS ONE CONTINUOUS ORGANELLE IN PANCREATIC EXOCRINE CELL OF MOUSE
- CYTOCHEMICAL LOCALIZATION OF CA^-ATPASE, H^+,K^+-ATPASE AND NA^+,K^+-ATPASE IN ACID-SECRETING PARIETAL CELL AND NON-SECRETING PARIETAL CELL
- CYTOCHEMICAL LOCALIZATION OF ADENYLATE CYCLASE AND GUANYLATE CYCLASE IN THE PARIETAL CELL OF GUINEA PIG GASTRIC GLAND
- ULTRACYTOCHEMICAL STUDIES OF ADENOSINE NUCLEOTIDASES IN AORTIC ENDOTHELIAL AND SMOOTH MUSCLE CELLS : Ca^-ATPase AND Na^+,K^+-ATPase
- CYTOCHEMISTRY OF HYDROGEN PEROXIDE PRODUCTION BY CULTURED ACTIVATED MACROPHAGES IN PHAGOCYTOSIS
- The Ultracytochemical Demonstration of Choline Acetylase Activity in the Nervous Tissue of Rat
- The Development of the Method for the Demonstration of NADPH-ferricyanide Reductase Activity and its Application in Several Tissues of Rats.
- Ultracytochemical Study of Glucose-6-Phosphatase Activity in Rat Hepatic Parenchymal Cells Treated with 2-Methyl-Dimethyl-Aminoazobenzene (2-MeDAB)
- ULTRACYTOCHEMICAL STUDIES ON THE DIURNAL CHANGE OF THE ENZYMATIC ACTIVITY IN VARIOUS TISSUES OF THE RAT, WITH SPECIAL REFERENCE TO THE MITOCHONDRIAL OXIDOREDUCTASE ACTIVITY
- ULTRACYTOCHEMICAL DEMONSTRATION OF NADPH-FERRICYANIDE REDUCTASE ACTIVITY