2-ヒドロキシ-3-スルホ-5-クロロアニリン-N-サリチリデンを用いるアルミニウムの蛍光フローインジェクション法による定量

概要

論文の詳細を見る
Four 2-hydroxyazomethine derivatives which have a sulfo and hydroxy groups in benzene rings have been synthesized to give higher water solubility and fluorometric sensitivity. Using FIA, their fluorescence properties were investigated. Since HSCS (the title compound) was found to be most sensitive it was used as a fluorometric reagent for aluminium. A dual channel FIA apparatus was used. An aliquot sample solution (80 μl) was injected into a carrier stream (distilled water) and was merged with a reagent stream containing 0.2% HSCS and 0.1 M acetate buffer (pH 6.0). The wavelength of the fluorometric spectrophotometer were set at Ex 420 nm and Em 505 nm. The calibration curve was linear over the range of 5 to 100 ng/ml of Al. The detection limit was 0.5 ng/ml (S/N=2). The relative standard deviations for 20 (n=5) and 100 (n=5) ng/ml of Al were 0.45 and 0.94%, respectively. Ga(III), Sc(III), Fe(III), Si(IV), Ca(II) and fluoride interfered with the determination.
社団法人日本分析化学会の論文
1991-07-05