フローインジェクション分析法による微量の亜硝酸及び硝酸イオンの定量

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A system has been developed for the determination of nitrite and nitrate ions by flow injection analysis. The flow lines were made from polytetrafluoroethylene (PTFE) tubing. For the nitrite determination a 650 *l of sample is injected into a stream of 0.04 % (w/v) p-aminoacetophenone (p-AAP) solution in 47 mM hydrochloric acid, and flows down a mixing coil (1 mmφ × 1 m) in a thermostated bath at 45 ℃. The mixture meets a stream of 0.11 % (w/v) m-phenylene-diamine dihydrochloride (m-PD) solution in 1.2 mM hydrochloric acid. After the mixing coil (1 mmφ × 1 m, 45 ℃), the absorbance at 456 nm is measured by a spectrophotometer with a 10-mm flow-through cell (8 *l) against water as reference. For the nitrate determination a 650 *l of sample is injected into 1.2 mM EDTA carrier solution (pH 9.8) and passed through a copperized cadmium column {3 mmφ × 70 mm, particle size: (0.5〜2) mm} to convert quantitatively nitrate to nitrite. Then, the carrier solution is merged into the stream of a mixed reagent solution of 0.02 % (w/v) p-AAP and 0.055% (w/v) m-PD in 24 mM hydrochloric acid. After the mixing coil (1 mmφ× 1 m, 45 ℃), the absorbance is measured at 456 nm. Sampling rate was 30 samples per hour. The relative standard deviations (n=10) were 0.97 % and 0.89 % for 10 *g/l and 30 *g/l of nitrite-nitrogen, respectively, and 0.95 % and 0.66 % for 0.1 mg/l and 0.3 mg/1 of nitrate-nitrogen, respectively. A detection limit was 0.4 *g/l and 2 *g/l for nitrite- and nitrate-nitrogen, respectively.
社団法人日本分析化学会の論文
1982-12-05

フローインジェクション分析法による微量の亜硝酸及び硝酸イオンの定量

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