スルホナゾIII-バリウム錯体を利用する蒸留酒中のエタノール含有量の吸光光度-フローインジェクション法

概要

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Sulfonazo III {2,7-Bis(ο-sulfophenylazo)-1,8-dihy-droxy-3,6-naphthalene disulfonic acid} reacts with barium to form a blue complex in a weak acidic solution. The absorption maximum of the complex lies at 640nm and the absorbances increase considerably with increasing the content of water miscible solvent, such as methanol, ethanol and acetone. This phenomenon was used to determine ethanol by FIA with spectrophotometric detection. An acetate buffer(0.01M, pH 3.5)containing 1.0×10^<-4>M Sulfonazo III and 4.0×10^<-5>M barium ion was delivered as a reagent solution. A sample solution(180μl)was injected into a carrier solution(distilled water). Both the reagent and carrier streams were pumped at a constant flow rate of 0.5 ml/min. A linear relationship between the peak heights and the ethanol concentrations was obtained up to 50v/v% of ethanol. The relative standard deviation was 0.5I and 0.87% for the determination of 10 and 30v/v% of ethanol, respectively(n=5). The proposed method was applied to the measurement of ethanol content in alcoholic beverages. The results on distilled alcoholic beverages agreed well with certified values.
社団法人日本分析化学会の論文
1990-10-05