糖-ホウ酸錯体の生成を利用するキャピラリー電気泳動緩衝液

概要

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The pH of electrophoretic buffer solutions is an important factor affecting the separation of analytical species by capillary electrophoresis. In pH adjustment, the use of electrolytes increases the ionic strength. The increased current against the high voltage (>10 kV) generates Joule heat and ruins the separation. Thus it is necessary to prepare buffer solutions without increasing ionic strength. The saccharide-borax system was found to be suitable for this purpose. Saccharide reacts with boric acid to form a dissociable complex, the pH varying from 4 to 9 easily. In this experiment, fructose was used as the saccharide and ten aminonaphthalenesulfonic acids (ANS) were selected as model compounds for separation. The pH dropped as fructose was added. By adding 600 mM fructose in 20 mM borax solution (pH 4.6), nine ANS were separated successfully.
社団法人日本分析化学会の論文
1995-03-05